Isolation and characterization of a new mutant allele of brachytic 2 maize gene
Isolation and characterization of a new mutant allele of brachytic 2 maize gene
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DOI：
10.1007/s11032-006-9073-7
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发表时间：
2007-09-01
影响因子：
3.1
通讯作者：
Landoni, Michela
中科院分区：
文献类型：
--
作者：
Pilu, Roberto;Cassani, Elena;Landoni, Michela
在玉米中已经描述了40多个单基因矮化突变体，然而，这些突变体中的大多数都会导致籽粒产量的大幅下降，因此，它们没有被用来提高对倒伏敏感的种质的作物产量。商业价值方面的例外是玉米突变体brachytic 2(BR2)。Br2基因于2003年被克隆，其编码的蛋白质最有可能参与生长素的极地运输。我们已经分离到一个新的与BR2基因座等位的短粒突变，并将其命名为BR2-23。对该突变体的鉴定表明，BR2突变不仅改变了之前报道的节间长度，而且还改变了叶片的结构。BR2-23/BR2-23杂合子在株高、穗位高和叶角方面有一个有用的中间表型，这表明这一效应可能在开发新的杂交种中得到利用。这个突变体似乎也是一个有用的工具，可以用来研究决定玉米株高和结构的复杂发育程序的转换点。
More than 40 monogenic dwarfing mutants have been described in maize; however, the majority of these lead to great reductions in grain yield and, consequently, they have not been used to enhance crop yield in germplasm that is sensible to lodging. An exception in terms of commercial value is the maize mutant brachytic 2 (br2). Br2 gene was cloned in 2003, and it encodes for a protein most probably involved in auxin polar transport. We have isolated a new brachytic mutation that is allelic to the br2 locus and denoted this novel mutant as br2-23. Characterization of this mutant revealed that the br2 mutation modified not only the length of the internodes, as previously reported, but the structure of the leaves as well. Br2-23/br2-23 heterozygotes have a useful intermediate phenotype in terms of plant height, ear height and leaf angle, suggesting a possible utilization of this effect in developing new hybrids. This mutant also appears to be an useful tool by which to study the switch points of the complex developmental program determining maize plant height and architecture.