Generating primary cultures of murine cardiac myocytes and cardiac fibroblasts to study viral myocarditis.
Generating primary cultures of murine cardiac myocytes and cardiac fibroblasts to study viral myocarditis.
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DOI：
10.1007/978-1-4939-2572-8_1
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Sherry B
中科院分区：
文献类型：
--
作者：
Sherry B
病毒可直接损伤心肌细胞和心脏成纤维细胞，导致心肌炎和心功能损害。心肌细胞和心脏成纤维细胞在支持病毒感染和产生保护性抗病毒反应方面表现出不同的能力。本章提供了小鼠心肌细胞和心脏成纤维细胞原代培养的详细方案，提供了一个强大的工具来探索确定对病毒性心肌炎的保护作用的细胞类型特异性反应。
Viruses can induce direct damage to cardiac myocytes and cardiac fibroblasts resulting in myocarditis and impaired cardiac function. Cardiac myocytes and cardiac fibroblasts display different capacities to support viral infection and generate a protective antiviral response. This chapter provides detailed protocols for generation and characterization of primary cultures of murine cardiac myocytes and cardiac fibroblasts, offering a powerful tool to probe cell type-specific responses that determine protection against viral myocarditis.