MPEG-DSPE polymeric micelle for translymphatic chemotherapy of lymph node metastasis
MPEG-DSPE polymeric micelle for translymphatic chemotherapy of lymph node metastasis
复制标题
MPEG-DSPE聚合物胶束用于淋巴结转移的经淋巴化疗
DOI：
10.1016/j.ijpharm.2015.03.074
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发表时间：
2015-06-20
影响因子：
5.8
通讯作者：
Liu, Min
中科院分区：
文献类型：
--
作者：
Li, Xue;Dong, Qing;Liu, Min
淋巴结转移是肿瘤形成的主要途径之一，并且难以将治疗浓度的化学治疗剂递送至淋巴结转移。本研究制备了甲基聚乙二醇-二硬脂酰磷脂酰乙醇胺/阿霉素（MPEG-DSPE/DOX）胶束，用于淋巴结转移瘤的治疗。制备的MPEG-DSPE/DOX胶束具有球形形态，粒径为20 +/-5nm。A375细胞对DOX和MPEG-DSPE/DOX胶束的摄取率分别为51.2%和88.7%。MPEG-DSPE/罗丹明B胶束对RAW264.7细胞的吞噬率比单独罗丹明B低17.2倍。皮下注射后，MPEG-DSPE胶束发生淋巴吸收，并在腘淋巴结中蓄积。与单纯DOX相比，MPEG-DSPE/DOX胶束可明显减轻注射部位皮下组织的损伤。我们建立了A375细胞裸鼠淋巴结转移模型。皮下注射后，MPEG-DSPE/DOX胶束组的腘淋巴结和髂淋巴结的重量均显著低于生理盐水组和DOX组。MPEG-DSPE/DOX胶束能有效杀伤腘、髂淋巴结肿瘤细胞。因此，MPEG-DSPE胶束是一种很有前途的治疗淋巴结转移的给药系统。(C)2015 Elsevier B. V.版权所有。
Lymph node metastasis is one of the major pathways for tumor formation and it is difficult to deliver chemotherapeutics at therapeutic concentrations to lymph node metastasis. This study prepared methyl poly(ethylene glycol)-distearoylphosphatidylethanolamine/doxorubicin (MPEG-DSPE/DOX) micelle for the treatment of lymph node metastasis. The MPEG-DSPE/DOX micelle prepared were of spherical morphology with a particle size of 20 +/- 5 nm. The uptake rates of DOX and MPEG-DSPE/DOX micelle by A375 cells were 51.2% and 88.7%, respectively. The phagocytosis rate of MPEG-DSPE/Rhodamine B micelle by RAW264.7 cells was 17.2-fold lower than for Rhodamine B alone. After subcutaneous injection, MPEG-DSPE micelle underwent lymphatic absorption and accumulated in popliteal lymph nodes. MPEG-DSPE/DOX micelle significantly alleviated damage to the subcutaneous tissue of the injection sites compared with DOX alone. We established a model of nude mice bearing lymph node metastasis of A375 cells. After subcutaneous injection, the weights of both the popliteal and iliac lymph nodes of the MPEG-DSPE/DOX micelle group were significantly lower than in the saline and DOX groups. MPEG-DSPE/DOX micelle effectively killed the tumor cells in popliteal and iliac lymph nodes. In conclusion, MPEG-DSPE micelle is a promising drug delivery system for the treatment of lymph node metastasis. (C) 2015 Elsevier B.V. All rights reserved.