Associations between measles vaccine immunity and single-nucleotide polymorphisms in cytokine and cytokine receptor genes
Associations between measles vaccine immunity and single-nucleotide polymorphisms in cytokine and cytokine receptor genes
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DOI：
10.1086/510596
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发表时间：
2007-01-01
影响因子：
6.4
通讯作者：
Jacobson, Robert M.
中科院分区：
文献类型：
--
作者：
Dhiman, Neelam;Ovsyannikova, Inna G.;Jacobson, Robert M.
背景。细胞因子是麻疹疫苗体液和细胞免疫的关键调节剂。与细胞因子水平差异相关的单核苷酸多态性（SNP）也应该影响麻疹疫苗诱导的免疫力。方法。我们对 118 名麻疹、腮腺炎、风疹疫苗接种受试者的 6 个细胞因子基因（白细胞介素 [IL]-2、IL-4、IL-10、IL-12A、IL-12B 和干扰素 [IFN]-gamma）及其受体（IL-2RA、IL-2RB、IL-4RA、IL-10RA、IL-10RB、IL-12RB1、 IL-12RB2 和 IFN-γ R)。使用chi(2) 检验和协方差分析确定SNP 与麻疹特异性抗体、淋巴细胞增殖和分泌细胞因子的关联。结果。我们发现 IL-2、IL-10 和 IL-12RB 基因中的 SNP 与麻疹疫苗诱导的免疫之间存在显着关联 (P < .05)。 IL-2基因内的IVS1-100G (rs2069762)和Ex2-34G (rs2069763) SNP与麻疹的高抗体和高淋巴增殖反应相关，而IL-10和IL-12R基因内的SNP与麻疹的低抗体和淋巴增殖反应相关。 IL-4RA 和 IL-12B 基因内的 SNP 在免疫反应测量中差异显着 (P < .05)。还发现 SNP 和分泌的细胞因子水平之间存在显着关联。结论。细胞因子和细胞因子受体基因中的特定 SNP 与麻疹疫苗接种免疫反应的变化显着相关。这些结果需要在更大的队列中进一步验证。
Background. Cytokines are key regulators of measles vaccine humoral and cellular immunity. Single-nucleotide polymorphisms (SNPs) that are associated with differences in cytokine levels should also influence measles vaccine induced immunity.Methods. We genotyped 118 measles-mumps-rubella-vaccinated subjects for SNPs from 6 cytokine genes (interleukin [IL]-2, IL-4, IL-10, IL-12A, IL-12B, and interferon [IFN]-gamma) and their receptors (IL-2RA, IL-2RB, IL-4RA, IL-10RA, IL-10RB, IL-12RB1, IL-12RB2, and IFN-gamma R). Associations of SNPs with measles-specific antibodies, lymphoproliferation, and secreted cytokines were determined using chi(2) tests and analyses of covariance.Results. We found significant associations (P < .05) between SNPs in the IL-2, IL-10, and IL-12RB genes and measles vaccine-induced immunity. The IVS1-100G (rs2069762) and the Ex2-34G (rs2069763) SNPs within the IL-2 gene were associated with high antibody and high lymphoproliferative responses, whereas SNPs within the IL-10 and IL-12R genes were associated with low antibody and lymphoproliferative responses to measles. SNPs within the IL-4RA and IL-12B genes varied significantly (P < .05) across immune response measures. Significant associations were also found between SNPs and secreted cytokine levels.Conclusions. Specific SNPs in the cytokine and cytokine receptor genes are significantly associated with variations in measures of the immune response to measles vaccination. These results need to be further validated in a larger cohort.