Fluorogenic Ion Sensing System Working in Water, Based on Stimulus-Responsive Copolymers Incorporating a Polarity-Sensitive Fluorophore
Fluorogenic Ion Sensing System Working in Water, Based on Stimulus-Responsive Copolymers Incorporating a Polarity-Sensitive Fluorophore
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DOI：
10.1021/ma071641i
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发表时间：
2007-11
期刊：
影响因子：
5.5
通讯作者：
Maki Onoda;S. Uchiyama;T. Ohwada
中科院分区：
文献类型：
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作者：
Maki Onoda;S. Uchiyama;T. Ohwada
我们开发了一种新型荧光（荧光关闭）离子传感器，该传感器基于包含极性敏感荧光团的刺激响应合成共聚物。在水溶液中结合目标离子后，这些大分子荧光传感器在功能温度下将其三维结构从开放形式改变为球状形式，这引起极性敏感荧光团周围微环境极性的变化。荧光团将这种环境极性变化转化为荧光信号。我们的聚合物传感器是通过选自四种共聚单体的共聚单体的无规共聚来制备的。首先，通过开发荧光 H+ 传感器来检查每种类型的共聚单体在聚合物传感器中的作用。接下来，开发了荧光 K+ 离子传感器，以证明这种“堆积”设计概念的通用性。最后，首次开发了在水中工作的荧光SO42-传感器。
We have developed fluorogenic (fluorescent off-on) ion sensors of a new type, based on stimulus-responsive synthetic copolymers incorporating a polarity-sensitive fluorophore. Upon binding a target ion in aqueous solution, these macromolecular fluorogenic sensors change their three-dimensional structure from an open form to a globular form at functional temperatures, and this induces a change of microenvironmental polarity around the polarity-sensitive fluorophore. The fluorophore transforms this environmental polarity change into a fluorescence signal. Our polymeric sensors were prepared by random copolymerization of comonomers selected from four types of comonomers. First, the role of each type of comonomer in the polymeric sensors was examined through the development of a fluorogenic H+ sensor. Next, a fluorogenic K+ ion sensor was developed in order to demonstrate the generality of this “buildup” design concept. Finally, a fluorogenic SO42- sensor working in water was developed for the first time.