Seasonal change in environmental DNA concentration of a submerged aquatic plant species
Seasonal change in environmental DNA concentration of a submerged aquatic plant species
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沉水植物物种环境 DNA 浓度的季节变化
DOI：
10.1086/704996
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发表时间：
2019
影响因子：
1.8
通讯作者：
Doi Hideyuki
中科院分区：
文献类型：
--
作者：
Matsuhashi Saeko;Minamoto Toshifumi;Doi Hideyuki
环境DNA（eDNA）方法越来越多地用于检测水生生物。为了优化自然环境中的调查效率，有必要了解目标物种eDNA浓度的季节变化，并确定eDNA浓度最高的季节和环境条件。近年来，eDNA方法已被开发用于检测水生植物物种，但eDNA浓度的季节变化仍然没有调查。许多水生植物一年四季在数量、大小和形状上都有相当大的变化。因此，它们的eDNA浓度可能沿着其物候而变化。通过测定日本农业池塘中黑藻（Hydrilla verticillata）的eDNA浓度，研究了黑藻eDNA浓度的季节变化。轮枝藻从5个池塘一年5×。该物种在冬季有一个休眠期，从春季到秋季有一个生长期。生长期的eDNA含量高于休眠期。使用eDNA进行物种检测的管理和保护调查应在eDNA浓度最高时进行，以最大限度地提高检测概率，从而提高调查效率。
Environmental DNA (eDNA) methods are increasingly used to detect aquatic organisms. To optimize survey efficiency in natural environments, it is necessary to understand the seasonal change in eDNA concentrations of target species and identify the season and environmental conditions in which eDNA concentrations are highest. Recently, eDNA methods have been developed to detect aquatic plant species, but the seasonal change in the eDNA concentrations remains uninvestigated. Many aquatic plants undergo considerable changes in abundance, size, and shape throughout the year. Therefore, their eDNA concentration may change along with their phenology. We investigated the seasonal change in the eDNA concentration of an aquatic submerged species,Hydrilla verticillata, in agricultural ponds in Japan by measuring the eDNA concentration ofH. verticillatafrom 5 ponds 5× in a year. This species has a dormant period during winter and a growth period from spring to autumn. The eDNA concentrations were higher during the growth period than during the dormant period. Management and conservation surveys that use eDNA for species detection should be done when eDNA concentrations are highest to maximize detection probabilities and therefore survey efficiency.