Thiamine primed defense provides reliable alternative to systemic fungicide carbendazim against sheath blight disease in rice (Oryza sativa L.)
Thiamine primed defense provides reliable alternative to systemic fungicide carbendazim against sheath blight disease in rice (Oryza sativa L.)
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DOI：
10.1016/j.plaphy.2012.05.003
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发表时间：
2012-08-01
影响因子：
6.5
通讯作者：
Kumar, J.
中科院分区：
文献类型：
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作者：
Bahuguna, Rajeev Nayan;Joshi, Rohit;Kumar, J.
以水稻纹枯病病原菌(Rhizoctonia solani AG-1a)为研究对象，评价了硫胺引发新的病原菌防御策略对水稻纹枯病的防治效果，并与全身杀菌剂多菌灵(BCM)进行了比较。用硫胺素(50 MM)和BCM(4 MM)处理半矮秆高产水稻品种Vasumati的种子。播种40d后，盆栽植株挑战接种纹枯病菌，研究硫胺素和BCM对水稻生长和产量性状的影响。较高的过氧化氢含量、总酚积累量、苯丙氨酸解氨酶(PAL)活性和超氧化物歧化酶(SOD)活性在硫胺处理下表现出较高的系统抗性水平，在攻击性病原菌侵染时这种抗性进一步增强。苯丙氨酸解氨酶(PAL)和锰超氧化物歧化酶(MnSOD)的高转录水平验证了硫胺素引发防御的模式。虽然在BCM治疗下观察到的疾病严重程度最低，但硫胺素产生了类似的结果，其疗效降低了18.12%。硫胺素处理显著降低了病原菌诱导的光合作用、气孔导度、叶绿素荧光、光合色素和硝酸还原酶(NR)活性的损失，同时增强了防御成分，对光合色素和硝酸还原酶(NR)活性影响不大。受病原菌侵染影响的生理性状被发现是表征植物在病原菌侵染下的反应的标志，并且在侵染的早期就可以检测到。这些发现为开发替代的、环境安全的策略来控制植物病害提供了一个新的范例。(C)2012年爱思唯尔·马森公司。版权所有。
A novel pathogen defense strategy by thiamine priming was evaluated for its efficacy against sheath blight pathogen, Rhizoctonia solani AG-1A, of rice and compared with that of systemic fungicide, carbendazim (BCM). Seeds of semidwarf, high yielding, basmati rice variety Vasumati were treated with thiamine (50 mM) and BCM (4 mM). The pot cultured plants were challenge inoculated with R. solani after 40 days of sowing and effect of thiamine and BCM on rice growth and yield traits was examined. Higher hydrogen peroxide content, total phenolics accumulation, phenylalanine ammonia lyase (PAL) activity and superoxide dismutase (SOD) activity under thiamine treatment displayed elevated level of systemic resistance, which was further augmented under challenging pathogen infection. High transcript level of phenylalanine ammonia lyase (PAL) and manganese superoxide dismutase (MnSOD) validated mode of thiamine primed defense. Though minimum disease severity was observed under BCM treatment, thiamine produced comparable results, with 18.12 per cent lower efficacy. Along with fortifying defense components and minor influence on photosynthetic pigments and nitrate reductase (NR) activity, thiamine treatment significantly reduced pathogen-induced loss in photosynthesis, stomatal conductance, chlorophyll fluorescence, NR activity and NR transcript level. Physiological traits affected under pathogen infection were found signatory for characterizing plant's response under disease and were detectable at early stage of infection. These findings provide a novel paradigm for developing alternative, environmentally safe strategies to control plant diseases. (C) 2012 Elsevier Masson SAS. All rights reserved.