Biochemical characterization of mitochondria from adult worms and plerocercoid larvae of Spirometra mansoni shows mixed populations of anaerobic and aerobic mitochondria
Biochemical characterization of mitochondria from adult worms and plerocercoid larvae of Spirometra mansoni shows mixed populations of anaerobic and aerobic mitochondria
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曼氏螺线虫成虫和多尾蚴幼虫线粒体的生化特征显示厌氧和需氧线粒体的混合群体
DOI：
10.1016/j.parint.2022.102695
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发表时间：
2023
影响因子：
1.9
通讯作者：
Mita Toshihiro
中科院分区：
文献类型：
--
作者：
Takamiya Shinzaburo;Nakamura Takeshi;Tsubokawa Daigo;Tsuji Naotoshi;Fukuda Koichi;Yamasaki Hiroshi;Fujimura Tsutomu;Taka Hikari;Kaga Naoko;Kobayashi-Tanabe Momoko;Matsuda Motoo;Mita Toshihiro
本文用电镜组织化学方法，对曼氏迭宫绦虫成虫和裂头蚴的线粒体进行了分离和原位观察，并对呼吸链进行了观察。虽然琥珀酸氧化酶的组成酶复合物的比活性在成人和plerocercoid线粒体中是相当相似的，琥珀酸氧化酶和NADH-FRD在成人线粒体中分别比plerocercoid线粒体高约4倍和25倍。通过高效液相色谱和质谱分析的醌分析表明，成虫和多尾虫的线粒体都含有玫瑰醌-10和泛醌-10，成虫的浓度分别为4.98和0.106 nmol mg− 1，多尾虫的浓度分别为0.677和0.137 nmol mg− 1。对成年线粒体琥珀酸氧化酶系统的抑制研究表明，它们同时具有对氰化物敏感和不敏感的琥珀酸氧化酶，后者产生过氧化氢。成人线粒体，当NADH被用作底物，显示出产生过氧化氢，和过氧化氢的生产下降到检测不到的水平，在富马酸盐的存在下。成熟节片的NADH-延胡索酸还原酶和细胞色素氧化酶的比活性显著高于未成熟节片和妊娠节片。等密度梯度离心分析和原位电镜组织化学显示，无论是成人和plerocercoid线粒体的呼吸功能和理化性质的异质性。成人和plerocercoid线粒体的生理意义进行了讨论，在其寄生栖息地的氧张力。
The mitochondria of adult and plerocercoid Spirometramansoniwere characterized in isolated mitochondria andin situby electron microscopic histochemistry with special attention to the respiratory chain. Although the specific activities of the constituent enzyme complexes of succinate oxidase are fairly similar in adult and plerocercoid mitochondria, those of succinate oxidase and NADH-FRD are approximately 4- and 25-fold higher in adult mitochondria than in plerocercoid mitochondria, respectively. Quinone analysis by high performance liquid chromatography and mass spectrometry showed that adult and plerocercoid mitochondria contained both rhodoquinone-10 and ubiquinone-10 at concentrations of 4.98 and 0.106 nmol mg−1for adult, and 0.677 and 0.137 nmol mg−1for plerocercoid, respectively. Inhibition studies on the succinate-oxidase system of adult mitochondria showed that they possessed both cyanide-sensitive and -insensitive succinate oxidases, the latter of which produces hydrogen peroxide. Adult mitochondria, when NADH was used as a substrate, were shown to produce hydrogen peroxide, and the production of hydrogen peroxide decreased to undetectable levels in the presence of fumarate. The specific activities of NADH-fumarate reductase and cytochromecoxidase were significantly higher in mature proglottids than in immature and gravid proglottids. Isopycnic density-gradient centrifugation analyses andin situelectron microscopic histochemistry revealed that both adult and plerocercoid mitochondria were heterogeneous in terms of respiratory function and physicochemical properties. The physiological significance of adult and plerocercoid mitochondria is discussed in relation to the oxygen tension of their parasitic habitats.