The LIN-29 transcription factor is required for proper morphogenesis of the Caenorhabditis elegans male tail.
The LIN-29 transcription factor is required for proper morphogenesis of the Caenorhabditis elegans male tail.
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DOI：
10.1006/dbio.1998.9063
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发表时间：
1999-02
影响因子：
2.7
通讯作者：
S. Euling;J. Bettinger;A. Rougvie
中科院分区：
文献类型：
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作者：
S. Euling;J. Bettinger;A. Rougvie
隐杆线虫基因lin-29编码一种锌指转录因子，这种转录因子是皮下细胞末梢分化和正常外阴形态发生所必需的。在这里，我们证明了lin-29也是雄性繁殖交配所必需的。研究表明，lin-29雄性可以进行早期交配行为，包括对雌雄同体接触和外阴定位的反应，但它们不进行通过针尖插入和精子转移的外阴附着步骤。与这一观察结果一致的是，我们发现lin-29突变体的针状体比野生型的针状体平均短43%，而其他雄性交配结构没有改变。在lin-29突变体中，在产生针状细胞后，当针状细胞形态发生在野生型雄性时，针状细胞发育出现了错误。我们发现LIN-29在野生型雄尾的许多细胞中积累，包括那些形成针状体的细胞。通过对遗传嵌合体的分析，我们证明了野生型针状体长度的形成需要AB.p谱系中的lin-29(+)，而AB.p谱系产生了针状体和其他雄性交配结构。我们的镶嵌分析还揭示了lin-29（+）在P1谱系中的作用，该谱系主要产生性肌、体细胞性腺细胞和体壁肌。
The Caenorhabditis elegans gene lin-29 encodes a zinc-finger transcription factor that is required for hypodermal cell terminal differentiation and proper vulva morphogenesis. Here we demonstrate that lin-29 is also required in males for productive mating. We show that lin-29 males can perform the early mating behaviors including response to hermaphrodite contact and vulva location, but they do not perform the subsequent steps of vulva attachment via spicule insertion and sperm transfer. Consistent with this observation, we found that lin-29 mutant spicules are on average 43% shorter than wild-type spicules while other male mating structures appear unaltered. In lin-29 mutants, spicule development goes awry after the generation of spicule cells, when spicule morphogenesis occurs in wild-type males. We show that LIN-29 accumulates in many cells of the wild-type male tail, including those that form the spicules. We demonstrate, through analysis of genetic mosaics, that the formation of wild-type-length spicules requires lin-29(+) in the AB.p lineage, the lineage that gives rise to the spicules and other male copulatory structures. Our mosaic analysis also reveals a role for lin-29(+) in the P1 lineage, which mainly produces sex muscles, cells of the somatic gonad, and body wall muscles.