BOUNDARY ANALYSIS OF SEDIMENTATION-VELOCITY EXPERIMENTS WITH MONODISPERSE AND PAUCIDISPERSE SOLUTES
BOUNDARY ANALYSIS OF SEDIMENTATION-VELOCITY EXPERIMENTS WITH MONODISPERSE AND PAUCIDISPERSE SOLUTES
复制标题
DOI：
10.1002/bip.1978.360170602
复制
发表时间：
1978-01-01
期刊：
影响因子：
2.9
通讯作者：
WEISCHET, WO
中科院分区：
文献类型：
--
作者：
VANHOLDE, KE;WEISCHET, WO
提出了一种方法，允许人们从单分散或寡分散溶质的沉降速度实验的边界计算沉降系数的分布。两种溶质的 S 值差异小至 20% 都可以解决。在不存在异质性和浓度依赖性效应的情况下，该分析还提供精度为 -10 至 +5% 的扩散系数值。对短 DNA 片段的模拟数据和超速离心实验的测试显示了该方法的价值和局限性。
A method is presented which allows one to calculate a distribution of sedimentation coefficients from the boundries of sedimentation‐velocity experiments with mono‐ or pauci‐disperse solutes. With two solutes differences inSvalue as small as 20% can be resolved. In absence of heterogeneity and concentration‐dependent effects, the analysis also provides values for the diffusion coefficient within an accuracy of −10 to +5%. Tests with both simulated data and ultracentrifugation experiments on short DNA fragments show the value and the limitations of the method.