Elevated 4-hydroxynonenal in ventricular fluid in Alzheimer's disease
Elevated 4-hydroxynonenal in ventricular fluid in Alzheimer's disease
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DOI：
10.1016/s0197-4580(97)00108-5
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发表时间：
1997-09-01
影响因子：
4.2
通讯作者：
Markesbery, WR
中科院分区：
文献类型：
--
作者：
Lovell, MA;Ehmann, WD;Markesbery, WR
4-羟基壬烯醛（4-HNE）是脂肪酸过氧化反应的醛类副产物，已被证明对培养的神经元具有毒性。鉴于越来越多的证据表明，氧化应激有助于阿尔茨海默病（AD）的神经退行性过程中，我们定量水平的自由和蛋白结合的4-HNE从19个AD受试者和13个对照组的高压液相色谱和斑点印迹免疫分析。发现AD受试者的脑室液中游离4-HNE水平与对照受试者相比显著升高（p = 0.0096）。这些结果表明AD脑中脂质过氧化增加，并表明4-HNE在神经退行性过程中的作用。(C)1997年爱思唯尔科学公司
4-Hydroxynonenal (4-HNE), an aldehyde by-product of the peroxidation of fatty acids, has been shown to have toxic properties for neurons in culture. In light of increasing evidence that oxidative stress contributes to the neurodegenerative process in Alzheimer's disease (AD), we quantified levels of free and protein-bound 4-HNE in the ventricular fluid from 19 AD subjects and 13 control subjects by high-pressure liquid chromotography and dot-blot immunoassay. Free 4-HNE levels were found to be significantly elevated in the ventricular fluid of AD subjects compared with control subjects (p = 0.0096). These results demonstrate increased lipid peroxidation in AD brain and suggest a role for 4-HNE in the neurodegenerative process. (C) 1997 Elsevier Science Inc.