The Aggregation of Brucella abortus Occurs Under Microaerobic Conditions and Promotes Desiccation Tolerance and Biofilm Formation.
The Aggregation of Brucella abortus Occurs Under Microaerobic Conditions and Promotes Desiccation Tolerance and Biofilm Formation.
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DOI：
10.2174/1874285801307010087
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sanjuan N
中科院分区：
文献类型：
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作者：
Almirón MA;Roset MS;Sanjuan N
牛种布氏杆菌主要在牛中引起布氏杆菌病。这种感染是通过摄入动物产品或直接接触受感染的物质传播给人类的。虽然布鲁氏菌的细胞内生活方式是很好的特点，其细胞外生存知之甚少。在自然界中，细菌的持久性与生物膜有关，在生物膜中，聚集的细胞受到保护免受逆境。牛种布氏杆菌在需氧条件下不能聚集，布氏杆菌的复制生态位以微需氧条件为特征，这一事实促使我们研究这种病原体在微需氧条件下聚集和生长在生物膜中的能力。结果表明，B.流产菌聚集并产生生物膜。即使在没有脂多糖-O抗原、鞭毛、转录调节因子VjbR或合成、转运和修饰环状β（1，2）葡聚糖的酶的情况下，聚集体也比脱水细胞更能耐受干燥、粘附和移位。
Brucella abortus causes brucellosis mainly in cattle. The infection is transmitted to humans by ingestion of animal products or direct contact with infected material. While the intracellular lifestyle of Brucella is well characterized, its extracellular survival is poorly understood. In nature, bacterial persistence is associated with biofilms, where aggregated cells are protected from adversity. The inability of Brucella abortus to aggregate under aerobiosis and that fact that the replicative niche of Brucella is characterized by microaerobic conditions prompted us to investigate the capacity of this pathogen to aggregate and grow in biofilms under microaerobiotic conditions. The results show that B. abortus aggregates and produces biofilms. The aggregates tolerate desiccation better than planktonic cells do, adhere and displace even in the absence of the lipopolysaccharide-O antigen, flagella, the transcriptional regulator VjbR, or the enzymes that synthesize, transport, and modify cyclic β (1,2) glucan.