Placental Transfer of Dengue Virus (DENV)-Specific Antibodies and Kinetics of DENV Infection-Enhancing Activity in Brazilian Infants
Placental Transfer of Dengue Virus (DENV)-Specific Antibodies and Kinetics of DENV Infection-Enhancing Activity in Brazilian Infants
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DOI：
10.1093/infdis/jiw143
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发表时间：
2016-07-15
影响因子：
6.4
通讯作者：
Marques, Ernesto T. A.
中科院分区：
文献类型：
--
作者：
Castanha, Priscila M. S.;Braga, Cynthia;Marques, Ernesto T. A.
背景。母胎转移登革热病毒（DENV）特异性抗体与婴儿期登革热的免疫发病机制有关。方法。在巴西东北部地区的登革热流行区建立了前瞻性出生队列。在 376 份配对的母体和脐带血样本中评估了 DENV 特异性免疫球蛋白 G (IgG) 和 DENV-1-4 血清型特异性中和抗体 (NAb) 水平。在该队列中登记的儿童的血清样本中确定了母体获得的 DENV 抗体增强活性的动力学。结果。母亲大多对单独的 DENV-3 (53.7%) 或与 DENV-4 (30.6%) 联合免疫。新生儿的 DENV 特异性 IgG、DENV-3 NAb 和 DENV-4 NAb 水平显着高于其各自的母亲。与对多种 DENV 血清型免疫的母亲相比，对单一血清型免疫的母亲转移的 DENV 特异性 IgG ( P = .02) 和 DENV-3 NAb ( P = .04) 水平更高。超过 90% 的儿童在 4 个月大时，母亲获得的 DENV-3 NAb 消失。 2月龄时检测到增强活性峰值（P
Background. Maternal-fetal transferred dengue virus ( DENV)-specific antibodies have been implicated in the immunopathogenesis of dengue during infancy. Methods. A prospective birth cohort was established in a dengue-endemic area in the Northeast Region of Brazil. DENV-specific immunoglobulin G ( IgG) and DENV-1-4 serotype-specific neutralizing antibody ( NAb) levels were assessed in 376 paired maternal and umbilical cord blood samples. The kinetics of enhancing activity by maternally acquired DENV antibodies was determined in serum samples from children enrolled in the cohort. Results. Mothers were mostly immune to DENV-3 alone ( 53.7%) or combined with DENV-4 ( 30.6%). Levels of DENV-specific IgG, DENV-3 NAbs, and DENV-4 NAbs were significantly higher in newborns than in their respective mothers. Mothers immune to a single serotype transferred greater levels of DENV-specific IgG ( P =.02) and DENV-3 NAbs ( P =.04) than mothers immune to multiple DENV serotypes. Maternally acquired DENV-3 NAbs disappeared in > 90% of the children by the age of 4 months. The peak enhancing activity was detected by the age of 2 months ( P