Analysis of motility parameters from paddlefish and shovelnose sturgeon spermatozoa
Analysis of motility parameters from paddlefish and shovelnose sturgeon spermatozoa
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DOI：
10.1111/j.1095-8649.2000.tb02148.x
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发表时间：
2000-06-01
影响因子：
2
通讯作者：
Rodina, M
中科院分区：
文献类型：
--
作者：
Cosson, J;Linhart, O;Rodina, M
经蒸馏水处理后，90% ~ 100%的白鲟可运动，速度为175 μ m s(-1)，鞭毛跳动频率为50 Hz，运动时间为406 min。同样，80-95%的铲鼻鲟（Scaphirhynchus platorynchus）精子在蒸馏水中稀释后立即运动，速度为200 μ m s(-1)，鞭毛跳动频率为48 Hz，运动周期为2-3分钟。在20 mM Tris-HCl （pH 8.2）和20 mM NaCl组成的游泳溶液中稀释精子后，大多数样品在激活后5 s内显示出100%的活力，鞭毛频率为50 Hz，速度高于蒸馏水。在这种游泳介质中，白鲟的运动时间延长了9分钟，鲟鱼的运动时间延长了5分钟，与蒸馏水相比，精子细胞出现鞭毛膜起泡或鞭毛尖端卷曲等损伤的比例较低。鞭毛波的形状在运动阶段发生改变，主要是由于鞭毛最接近头部的部分受到限制。两种精子均有全细胞的旋转运动。游泳介质与蒸馏水、白鲟与铲鼻鲟精子速度差异显著。(C) 2000年不列颠群岛渔业协会。
Ninety to 100% of paddlefish Polyodon spathula were motile just after transfer into distilled water, with a velocity of 175 mu m s(-1), a flagellar beat frequency of 50 Hz and motility lasting 406 min. Similarly. 80-95% of shovelnose sturgeon Scaphirhynchus platorynchus spermatozoa were motile immediately when diluted in distilled water, with a velocity of 200 mu m s(-1), a flagellar beat frequency of 48 Hz and a period of motility of 2-3 min. In both species, after sperm dilution in a swimming solution composed of 20 mM Tris-HCl (pH 8.2) and 20 mM NaCl a majority of the samples showed 100% motility of spermatozoa with flagella beat-frequency of 50 Hz within the 5 s following activation and a higher velocity than in distilled water. In such a swimming medium, the time of motility was prolonged up to 9 min for paddlefish and 5 min for sturgeon and a lower proportion of sperm cells had damage such as blebs of the flagellar membrane or curling of the flagellar tip, compared with those in distilled water. The shape of the flagellar waves changed during the motility phase, mostly through a restriction at the part of the flagellum most proximal to the head. A rotational movement of whole cells was observed for spermatozoa of both species. There were significant differences in velocity of spermatozoa between swimming media and distilled water and between paddlefish and shovelnose sturgeon. (C) 2000 The Fisheries Society of the British Isles.