Detection of the molecular abnormality in chronic myeloid leukemia by use of the polymerase chain reaction.
Detection of the molecular abnormality in chronic myeloid leukemia by use of the polymerase chain reaction.
复制标题
使用聚合酶链式反应检测慢性粒细胞白血病的分子异常。
作者：
Alexander Dobrovic;Kevin J. Trainor;Alexander A. Morley
以慢性粒细胞白血病（CML）的mRNA为起始材料，采用改良的聚合酶链反应（PCR）技术，检测CML的bcr-abl易位特征。扩增跨越bcr-abl连接的序列是通过使用所有20例典型CML患者的外周血细胞，1例可能继发于CML的急性淋巴细胞白血病患者和2个CML患者的细胞系获得的。mRNA中bcr外显子3的存在由扩增序列的大小确定;它存在于14例患者中，在7例患者中缺失。每1，000个非白血病细胞中可容易地检测到一个白血病细胞，从而表明该方法的高灵敏度。这项技术在CML的诊断和治疗过程中具有常规价值。
The bcr-abl translocation characteristic of chronic myeloid leukemia (CML) was detected by the polymerase chain reaction (PCR) modified to use mRNA as the starting material. Amplification of a sequence spanning the bcr-abl junction was obtained by using peripheral blood cells from all of 20 patients with classic CML, one patient with acute lymphoblastic leukemia probably secondary to CML, and two cell lines derived from patients with CML. The presence of bcr exon 3 in the mRNA was determined from the size of the amplified sequence; it was present in 14 and absent in seven patients. One leukemic cell per 1,000 nonleukemic cells could be readily detected, thus indicating the great sensitivity of the method. This technique is of routine value in CML both for diagnosis and for following the course of treatment.
处于急变危象的 CML 患者的断点位于 BCR 的特定区域。
影响因子：
20.3
作者：
Schaefer-Rego,K;Dudek,H;Popenoe,D;Arlin,Z;Mears,JG;Bank,A;Leibowitz,D
通讯作者：
Leibowitz,D