Fosfomycin and plasmidic resistance.
Fosfomycin and plasmidic resistance.
复制标题
磷霉素和质粒抗性。
DOI：
10.1159/000222039
复制
发表时间：
1977
期刊：
影响因子：
3.3
通讯作者：
T. Cañedo
中科院分区：
文献类型：
--
作者：
F. Baquero;M. López;A. Valls;T. Cañedo
将60株革兰氏阴性杆菌的磷霉素抗性菌株用作受体细胞E. coliK12，耐萘啶酸。交配后，耐磷霉素E.在含有萘啶酸和磷霉素的选择平板上生长的大肠杆菌K2菌落从未超过受体菌株磷霉素抗性的正常突变率。在65%的实验中，质粒对其他抗菌剂的耐药性转移到了E。coli K12，但从未伴随明显的磷霉素耐药性。受体菌株的高正常突变率是检测磷霉素抗性质粒性质的主要问题。根据我们关于这一事实的标准，我们无法证实磷霉素耐药性的质粒性质。
60 fosfomycin-resistant strains of gram-negative bacilli are submitted to conjugation experiments using as recipient cell E. coli K12 which is nalidixic acid resistant. After mating, the number of fosfomycin-resistant E. coli K2 colonies growing on selection plates containing nalidixic acid and fosfomycin never surpassed the normal rate of mutation for fosfomycin-resistance of the recipient strain. In 65% of the experiments, plasmidic resistance to other antimicrobials was transferred to E. coli K12, but was never accompanied by demonstrable fosfomycin resistance. High rate of normal mutation of recipient strain is signaled as the main problem for detecting the plasmidic nature of fosfomycin resistance. With our criteria regarding this fact we have been unable to confirm the plasmidic nature of fosfomycin resistance.