Analysis of azole fungicides in fish muscle tissues: Multi-factor optimization and application to environmental samples
Analysis of azole fungicides in fish muscle tissues: Multi-factor optimization and application to environmental samples
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鱼肌肉组织中唑类杀菌剂的分析：多因素优化及其在环境样品中的应用
DOI：
10.1016/j.jhazmat.2016.11.024
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发表时间：
2016
影响因子：
13.6
通讯作者：
Zongwei Cai
中科院分区：
文献类型：
--
作者：
Yuanhong Zhong;Zhi-Feng Chen;Shuang-Shuang Liu;Xiaoxin Dai;Xinping Zhu;Guangming Zheng;Shugui Liu;Guoguang Liu;Zongwei Cai
据报道，唑类杀菌剂可在鱼类组织中积累。本研究建立了一种高效液相色谱-串联质谱联用超声提取、固液净化、液液萃取、固相萃取（SPE）富集纯化的测定鱼类肌肉样品中唑类杀菌剂的方法。根据非统计分析和统计分析的结果，确定乙酸乙酯、伯仲胺（PSA）和混合模式阳离子交换滤筒（MCX）分别为最佳萃取溶剂、清洁吸附剂和SPE滤筒。采用内标法对不同鱼类肌肉基质的加样回收率（81.7 ~ 104%）和基质效应（- 6.34 ~ 7.16%）进行了校正。方法定量限为0.07 ~ 2.83 ng/g。该优化方法成功地应用于北江及其支流野鱼肌肉样品中目标物的测定。在鱼类肌肉组织中检测到克莉巴唑、克霉唑和多菌灵三种唑类杀菌剂的ppb水平。因此，该分析方法具有实用性，适用于进一步阐明野生鱼类中唑类杀菌剂的污染情况。
Azole fungicides have been reported to be accumulated in fish tissue. In this study, a sensitive and robust method using high-performance liquid chromatography-tandem mass spectrometry combined with ultrasonic extraction, solid-liquid clean-up, liquid–liquid extraction and solid-phase extraction (SPE) for enrichment and purification have been proposed for determination of azole fungicides in fish muscle samples. According to the results of non-statistical analysis and statistical analysis, ethyl acetate, primary secondary amine (PSA) and mixed-mode cation exchange cartridge (MCX) were confirmed as the best extraction solvent, clean-up sorbent and SPE cartridge, respectively. The satisfied recoveries (81.7–104%) and matrix effects (−6.34–7.16%), both corrected by internal standards, were performed in various species of fish muscle matrices. Method quantification limits of all azoles were in the range of 0.07–2.83 ng/g. This optimized method was successfully applied for determination of the target analytes in muscle samples of field fish from Beijiang River and its tributaries. Three azole fungicides including climbazole, clotrimazole and carbendazim were detected at ppb levels in fish muscle tissues. Therefore, this analytical method is practical and suitable for further clarifying the contamination profiles of azole fungicides in wild fish species.