THE BIPHASIC OPENING OF THE BLOOD-BRAIN-BARRIER TO PROTEINS FOLLOWING TEMPORARY MIDDLE CEREBRAL-ARTERY OCCLUSION
THE BIPHASIC OPENING OF THE BLOOD-BRAIN-BARRIER TO PROTEINS FOLLOWING TEMPORARY MIDDLE CEREBRAL-ARTERY OCCLUSION
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DOI：
10.1007/bf00688633
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发表时间：
1985-01-01
影响因子：
12.7
通讯作者：
KLATZO, I
中科院分区：
文献类型：
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作者：
KUROIWA, T;TING, P;KLATZO, I
本实验观察了猫大脑中动脉(MCA)阻断1h后血脑屏障(BBB)的行为变化。用氢清除法测定尾状核和大脑皮质局部脑血流量(RCBF)。伊文思蓝(EB)示踪法和免疫组织化学过氧化物酶-抗过氧化物酶(PAP)法检测血脑屏障。大脑中动脉闭塞松解后，血脑屏障有两个开口，由不应期隔开。第一次开放发生在再循环后不久；这与缺血期rCBF低于15ml/100g/min有关，并且在MCA闭塞解除后立即出现明显的反应性充血。给猫注射示踪剂前30分钟未见EB渗漏，再循环后3小时处死猫，提示血脑屏障不应期，但动物的rCBF值更低(6.+-)。1ml/100g/min)，再循环后均出现明显充血。PAP染色证实了这些动物以前发生过血脑屏障开放。死前30min注射EB的猫在再循环后5h和72h出现血脑屏障的第二次开放，阻断时rCBF低于15ml/100g/min，随后出现明显的反应性充血。阻断期间rCBF>15ml/100g/min且未显示明显反应性充血的猫，在任何时候均未见EB渗出。
The behavior of the blood-brain barrier (BBB) was studied in cats following release after 1-h middle cerebral artery (MCA) occlusion. The regional cerebral blood flow (rCBF) was determined by hydrogen clearance method in the caudate nucleus and the cerebral cortex. The BBB was assayed with Evans blue (EB) tracer and by immunohistochemical peroxidase-antiperoxidase (PAP) method. Following release of MCA occlusion, there were two openings of the BBB, separated by a refractory period. The first opening, occurred shortly after recirculation; this was associated with rCBF below 15 ml/100 g/min during the ischemic period and a pronounced reactive hyperemia promptly following release of MCA occlusion. A refractory period of the BBB was indicated by the absence of EB leakage in cats injected with the tracer 30 min before killing at 3 h after recirculation, although the rCBF values in these animals were even lower (6 .+-. 1 ml/100 g/min) during occlusion, and all of them showed a pronounced hyperemia after recirculation. The occurrence of the previous BBB opening in these animals was confirmed by the PAP staining. The second opening of the BBB was observed at 5 and 72 h after recirculation in cats which were injected with EB 30 min before killing, and which showed rCBF below 15 ml/100 g/min during occlusion, followed by a pronounced reactive hyperemia. No EB extravasations were observed at any time in cats in which the rCBF during occlusion was above 15 ml/100 g/min and which failed to show a marked reactive hyperemia.