Selective extraction of antioxidants with molecularly imprinted polymers
Selective extraction of antioxidants with molecularly imprinted polymers
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DOI：
10.1016/j.aca.2003.08.033
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发表时间：
2003-09
影响因子：
6.2
通讯作者：
O. Brüggemann;A. Visnjevski;R. Burch;P. Patel
中科院分区：
文献类型：
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作者：
O. Brüggemann;A. Visnjevski;R. Burch;P. Patel
分子印迹技术可用于生成特定的人工聚合物受体，即高亲和力的固定相，正如肽和许多其他食品成分所显示的那样。在这项工作中，聚合物被三种典型的抗氧化剂，丁基羟基甲苯（BHA），丁基羟基甲苯（BHT）和没食子酸丙酯（PG）分子印迹。色谱评价结果显示，与非特异性对照聚合物（CP）相比，印迹聚合物（MIP）具有特异性。对于BHA印迹聚合物和各自的BHA分析物，α=kBHA-MIP ' /kCP ' =1.12，而BHT-MIP具有较高的选择性，α=kBHT-MIP ' /kCP ' =1.47。使用PG选择性MIP作为分析物，α=kPG-MIP ' /kCP ' =1.24。此外，还介绍了基于MIP和CP相的提取方法的结果，例如，当使用甲醇或乙腈作为分析溶剂时，BHA-MIP与CP相相比具有更高的选择性，而在乙腈中进行提取时，PG-MIP则具有更高的选择性。
Molecular imprinting technology can be used to generate specific artificial polymeric receptors, i.e., high affinity stationary phases, as already shown for peptides and many other food ingredients. In this work, polymers have been molecularly imprinted with three exemplary antioxidants, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and propyl gallate (PG). Results of chromatographic evaluations are presented, demonstrating the specificity of the imprinted polymers (MIP) compared to non-specific control polymers (CP). For a BHA imprinted polymer and the respective analyte BHA a separation factor of α=kBHA-MIP′/kCP′=1.12 was found, whereas for the BHT-MIP a higher selectivity was determined with α=kBHT-MIP′/kCP′=1.47. Using the PG selective MIP for PG as analyte a value of α=kPG-MIP′/kCP′=1.24 could be achieved. Furthermore, results of extraction procedures based on MIP and CP phases are presented, demonstrating for instance a higher selectivity of the BHA-MIP in comparison with its CP when using methanol or acetonitrile as analyte solvent, and of the PG-MIP when performing the extraction in acetonitrile.