Photobiomodulation therapy improves both inflammatory and fibrotic parameters in experimental model of lung fibrosis in mice
Photobiomodulation therapy improves both inflammatory and fibrotic parameters in experimental model of lung fibrosis in mice
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DOI：
10.1007/s10103-017-2281-z
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发表时间：
2017-11-01
影响因子：
2.1
通讯作者：
Lino-dos-Santos-Franco, Adriana
中科院分区：
文献类型：
--
作者：
Brochetti, Robson Alexandre;Leal, Mayara Peres;Lino-dos-Santos-Franco, Adriana
肺纤维化（Lung fibrosis，LF）是一种以肺实质进行性病变、炎性浸润和间质纤维化为特征的慢性进行性肺部疾病。它是由过度的胶原蛋白沉积和其他细胞基质成分形成的，导致肺泡结构的严重变化。鉴于目前缺乏有效的治疗方法，本研究的目的是探讨光生物调节疗法（PBMT）对PF发生的影响。为此，我们使用经博莱霉素诱导的LF的C57 BL 6小鼠（1.5 U/kg），诱导14天后，用PBMT治疗小鼠，每天1次，持续8天（波长660 +/- 20 nm，功率100 mW，辐射曝光量5 J/cm（2），辐照度33.3 mW/cm（2），光斑尺寸2.8 cm（2），总能量15 J，辐照时间：150 s），并评价有或无PBMT的炎症和纤维化参数。我们的研究结果表明，PBMT显着减少肺泡腔中的炎性细胞的数量，胶原蛋白的产生，间质增厚，静态和动态肺弹性。此外，我们观察到培养物中肺细胞释放的IL-6 e CXCL 1/KC水平降低以及培养物中成纤维细胞释放的CXCL 1/KC水平降低。我们可以得出结论，PBMT改善了炎症和纤维化参数，显示出经济且无副作用的有前景的治疗。
Lung fibrosis (LF) is a chronic and progressive lung disease characterized by pulmonary parenchyma progressive lesion, inflammatory infiltration, and interstitial fibrosis. It is developed by excessive collagen deposition and other cellular matrix components, resulting in severe changes in the alveolar architecture. Considering the absence of effective treatment, the aim of this study was to investigate the effect of photobiomodulation therapy (PBMT) on the development of PF. For this purpose, we used C57BL6 mice subjected to induction of LF by bleomycin administration (1.5 U/kg) by orotracheal route and, after 14 days of the induction, mice were treated with PBMT applied to the thorax 1x/day for 8 days (wavelength 660 +/- 20 nm, power 100 mW, radiant exposure 5 J/cm(2), irradiance 33.3 mW/cm(2), spot size 2.8cm(2), total energy 15 J, time of irradiation: 150 s) and inflammatory and fibrotic parameters were evaluated with or without PBMT. Our results showed that PBMT significantly reduced the number of inflammatory cells in the alveolar space, collagen production, interstitial thickening, and static and dynamic pulmonary elastance. In addition, we observed reduced levels of IL-6 e CXCL1/KC released by pneumocytes in culture as well as reduced level of CXCL1/KC released by fibroblasts in culture. We can conclude that the PBMT improves both inflammatory and fibrotic parameters showing a promising therapy which is economical and has no side effects.