PDF receptor signaling in Caenorhabditis elegans modulates locomotion and egg-laying
PDF receptor signaling in Caenorhabditis elegans modulates locomotion and egg-laying
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DOI：
10.1016/j.mce.2012.05.001
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发表时间：
2012-09-25
影响因子：
4.1
通讯作者：
Schoofs, Liliane
中科院分区：
文献类型：
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作者：
Meelkop, Ellen;Temmerman, Liesbet;Schoofs, Liliane
在秀丽隐杆线虫中，pdfr-1 编码促胰液素受体家族的三种受体。这些 G 蛋白偶联受体由三种神经肽（色素分散因子 1a、1b 和 2）激活，它们由 pdf-1 和 pdf-2 编码。我们通过诱变筛选分离出 PDF 受体功能丧失等位基因 (lst34)，并表明 PDF 信号系统参与运动和产卵。我们证明 pdfr-1 突变体表型复制了 pdf-1 突变体的有缺陷的运动行为，并且 pdf-1 和 pdf-2 表现相反。所有三种 PDF 受体剪接变体都参与运动行为的调节。细胞特异性救援实验表明，这种 pdf 介导的行为是由神经元而不是体壁肌肉调节的。我们还表明，pdf-1 和 pdf-2 突变体的产卵模式受到影响，但 pdfr-1 突变体的产卵模式不受影响，这表明 PDF 系统在产卵调节中具有新的作用。 (c) 2012 Elsevier Ireland Ltd. 保留所有权利。
In Caenorhabdiris elegans, pdfr-1 encodes three receptors of the secretin receptor family. These G protein-coupled receptors are activated by three neuropeptides, pigment dispersing factors 1a, 1b and 2, which are encoded by pdf-1 and pdf-2. We isolated a PDF receptor loss-of-function allele (lst34) by means of a mutagenesis screen and show that the PDF signaling system is involved in locomotion and egg-laying. We demonstrate that the pdfr-1 mutant phenocopies the defective locomotor behavior of the pdf-1 mutant and that pdf-1 and pdf-2 behave antagonistically. All three PDF receptor splice variants are involved in the regulation of locomotor behavior. Cell specific rescue experiments show that this pdf mediated behavior is regulated by neurons rather than body wall muscles. We also show that egg-laying patterns of pdf-1 and pdf-2 mutants are affected, but not those of pdfr-1 mutants, pointing to a novel role for the PDF-system in the regulation of egg-laying. (c) 2012 Elsevier Ireland Ltd. All rights reserved.