Electro-Acupuncture Alleviates Cisplatin-Induced Anorexia in Rats by Modulating Ghrelin and Monoamine Neurotransmitters
Electro-Acupuncture Alleviates Cisplatin-Induced Anorexia in Rats by Modulating Ghrelin and Monoamine Neurotransmitters
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DOI：
10.3390/biom9100624
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发表时间：
2019-10-01
期刊：
影响因子：
5.5
通讯作者：
Kang, Ki Sung
中科院分区：
文献类型：
--
作者：
Baek, Ji Yun;Tuy An Trinh;Kang, Ki Sung
厌食症在癌症患者中很常见，主要是化疗的副作用。电针（EA）对改善癌症相关症状的作用已在动物模型和临床试验中进行了研究。本研究的目的是确定最佳条件下应用EA缓解厌食症，其次是影响其治疗的分子机制的研究。雄性Wistar大鼠腹腔注射顺铂诱导厌食，然后电针腹正中线中央穴位CV12。在整个研究期间，每天测量体重和摄食量。采用高效液相色谱-电子捕获检测器（HPLC-ECD）对血浆单胺类神经递质进行定量分析。用ELISA试剂盒阐明胃肠激素浓度。进行RT-qPCR以评估生长激素释放肽（GHRL）、神经肽Y（NPY）和阿黑皮素原的mRNA表达。Western blotting法检测孤束核c-Fos的表达。电针缓解大鼠厌食的最佳条件为强度1单位、频率10 Hz。电针CV12可降低血浆单胺类神经递质5-羟色胺、5-羟吲哚乙酸、多巴胺和去甲肾上腺素的水平，并刺激GHRL和NPY的表达，减轻顺铂诱导的大鼠厌食症。电针CV12对顺铂所致大鼠厌食症有治疗作用。
Anorexia is common in patients with cancer, mostly as a side effect of chemotherapy. The effect of electro-acupuncture (EA) on ameliorating cancer-related symptoms have been studied in animal models and in clinical trials. The aim of this study was to determine optimal conditions for the application of EA to alleviate anorexia, followed by the study of molecular mechanisms affecting its therapeutics. Anorexia was induced in male Wistar rats by injecting cisplatin, which was then followed by EA treatment at CV12, the acupuncture point located in the center of the abdominal midline. Body weight and food intake were measured daily throughout the duration of the study. The levels of monoamine neurotransmitters in the plasma were quantitatively analyzed by HPLC-ECD. Gastrointestinal hormone concentrations were elucidated with ELISA kits. RT-qPCR was performed to evaluate the mRNA expression of ghrelin (GHRL), neuropeptide Y (NPY), and pro-opiomelanocortin. The expression of c-Fos in the nucleus tractus solitarii was detected using western blotting analysis. The optimal conditions of EA to alleviate anorexia in rats was determined to be 1 unit for intensity and 10 Hz for frequency. EA treatment at CV12 reduced the levels of plasma monoamine neurotransmitters 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, dopamine, and norepinephrine; as well as stimulated the expression of GHRL and NPY to alleviate cisplatin-induced anorexia in rats. EA stimulation at CV12 could be used to treat cisplatin-induced anorexia in rats.