Epistatic effect between ACACA and FABP2 gene on abdominal fat traits in broilers
Epistatic effect between ACACA and FABP2 gene on abdominal fat traits in broilers
复制标题
DOI：
10.1016/s1673-8527(09)60070-9
复制
发表时间：
2010-08-01
影响因子：
5.9
通讯作者：
Li, Hui
中科院分区：
文献类型：
--
作者：
Hu, Guo;Wang, Shouzhi;Li, Hui
上位性通常被定义为两个或多个基因或其mRNA或蛋白质产物之间的相互作用，以影响单个性状。实验证据表明，上位性在确定家畜复杂性状的遗传结构中可能是重要的。乙酰辅酶A羧化酶α (ACACA)和脂肪酸结合蛋白2 （FABP2）都是脂肪生成和运输的关键因子。它们可能在生长鸡腹部脂肪组织的体重变异性中起关键作用。本研究在不同腹部脂肪含量选择的2个肉鸡品系中，检测到ACACA基因中C . 2292g > A和FABP2基因中C .- 561a > C的多态性。分析了两个snp在腹脂重（AFW）和腹脂率（AFP）上的上位性。在第9代和第10代瘦系中，这两个snp之间的加性x加性上位性成分在AFW和AFP上均显著或极显著；而与肥胖系AFW或AFP均无显著相关性。同时，在两代或两系中没有发现任何其他显著的上位性成分。这两个snp之间存在显著的上位性效应，可能部分是由于这两个实验品系的腹部脂肪性状在强人工选择的作用下被极大地改变了。结果表明，瘦肉鸡系和肥肉鸡系的上位模式可能不同。本研究结果有助于进一步了解影响肉鸡腹部脂肪含量表型变异的候选基因之间的遗传相互作用。
Epistasis is generally defined as the interaction between two or more genes or their mRNA or protein products to influence a single trait. Experimental evidence suggested that epistasis could be important in the determination of the genetic architecture of complex traits in domestic animals. Acetyl-coenzyme A carboxylase alpha (ACACA) and fatty acid binding protein 2 (FABP2) are both key factors of lipogenesis and transport. They may play a crucial role in the weight variability of abdominal adipose tissue in the growing chicken. In this study, the polymorphisms of c.2292G > A in ACACA and c.-561A > C in FABP2 were detected among individuals from two broiler lines which were divergently selected for abdominal fat content. Epistasis between the two SNPs on abdominal fat weight (AFW) and abdominal fat percentage (AFP) was analyzed. The additive x additive epistatic components between these two SNPs were found significant or suggestively significant on both AFW and AFP in lean lines of the 9th and 10th generation; whereas, it was not significantly associated with either AFW or AFP in fat lines. At the same time, there were not any other significant epistatic components found in both generations or in both lines. Significant epistatic effects between these two SNPs found only in the lean lines could partly be due to the fact that the abdominal fat traits in these two experimental lines have been greatly modified by strong artificial selection. The results suggested that the epistasis mode may be different between the lean and fat chicken lines. Our results could be helpful in further understanding the genetic interaction between candidate genes contributing to phenotypic variation of abdominal fat content in broilers.