MIC-100, a new system for high-throughput phenotyping of instantaneous leaf photosynthetic rate in the field
MIC-100, a new system for high-throughput phenotyping of instantaneous leaf photosynthetic rate in the field
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MIC-100，一种用于田间瞬时叶片光合速率高通量表型分析的新系统
DOI：
10.1071/fp21029
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发表时间：
2021
影响因子：
3
通讯作者：
Shunsuke Adachi
中科院分区：
文献类型：
--
作者：
Yu Tanaka;Kazuki Taniyoshi;Ayumu Imamura;Ryo Mukai;Shun Sukemura;Kazuma Sakoda;Shunsuke Adachi
光合作用主要发生在植物叶片中，是全球碳循环和作物生产的基本过程。利用叶片光合能力的自然遗传变异是满足作物日益增长的需求的一个有前途的策略。本研究报告了新开发的光合作用测量系统MIC-100，具有较高的吞吐量，用于测量瞬时光合速率在现场。MIC-100是建立在封闭系统的基础上，直接检测叶室中的CO2吸收。使用大豆（Glycine max L.）（Merr.））和水稻（Oryza sativa L.）在田间条件下生长。在大多数情况下，同一叶片重复测量的变异系数（CV）小于0.1。MIC-100模型测定的光合速率与气体交换系统测定的光合速率呈显著相关（R2 = 0.93-0.95）。MIC-100的测量吞吐量明显大于现场条件下的传统开放式气体交换系统。虽然MIC-100仅检测给定环境下的瞬时光合速率，但本研究表明，MIC-100能够粗略评估田间生长的大规模植物种群内的叶片光合作用。
Photosynthesis occurs mainly in plant leaves and is a fundamental process in the global carbon cycle and in crop production. The exploitation of natural genetic variation in leaf photosynthetic capacity is a promising strategy to meet the increasing demand for crops. The present study reports the newly developed photosynthesis measurement system ‘MIC-100,’ with a higher throughput for measuring instantaneous photosynthetic rate in the field. MIC-100 is established based on the closed system and directly detects the CO2 absorption in the leaf chamber. The reproducibility, accuracy, and measurement throughput of MIC-100 were tested using soybean (Glycine max L. (Merr.)) and rice (Oryza sativa L.) grown under field conditions. In most cases, the coefficient of variance (CV) for repeated-measurements of the same leaf was less than 0.1. The photosynthetic rates measured with the MIC-100 model showed a significant correlation (R2 = 0.93–0.95) with rates measured by a widely used gas-exchange system. The measurement throughput of the MIC-100 is significantly greater than that of conventional open gas-exchange systems under field conditions. Although MIC-100 solely detects the instantaneous photosynthetic rate under a given environment, this study demonstrated that the MIC-100 enables the rough evaluation of leaf photosynthesis within the large-scale plant populations grown in the field.