Histological scoring of articular cartilage alone provides an incomplete picture of osteoarthritic disease progression
Histological scoring of articular cartilage alone provides an incomplete picture of osteoarthritic disease progression
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DOI：
10.14670/hh-25.291
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发表时间：
2010-03-01
影响因子：
2
通讯作者：
Jomha, N. M.
中科院分区：
文献类型：
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作者：
Barley, R. D. C.;Bagnall, K. M.;Jomha, N. M.
目的：确定在关节软骨（AC）的既定组织学分级中是否存在疾病进展的分子亚类。研究方法：根据从18例膝关节置换术中获得的AC切片的H & E和番红-O染色，确定了30个样本，范围从Mankin评分系统1级到5级。对石蜡包埋的AC样品的连续切片进行II型胶原和聚集蛋白聚糖的免疫组织化学（IHC）分析。研究人员对五个Mankin评分系统等级的六个AC样本进行了检查。结果：在所有五个组织学等级的AC样本中，II型胶原和聚集蛋白聚糖沉积存在显著的IHC差异。II型胶原和聚集蛋白聚糖的IHC差异范围随组织学分级的增加而增加。在MG-3 AC中观察到II型胶原沉积模式的变化，这与II型胶原代谢的转换一致。结论：II型胶原和聚集蛋白聚糖的IHC染色可以识别AC的组织学分级内的差异，这些差异与分子亚类的存在一致。即使在最低组织学分级中也可检测到这些差异;因此，使用IHC染色可进一步增强和细化早期骨关节炎（OA）中AC恶化的评分。此外，聚集蛋白聚糖和II型胶原蛋白沉积模式的变化表明，它们可用于监测OA进展中的关键分子事件。这些发现也强调了制定IHC评分标准的必要性。
Purpose: To ascertain whether molecular subcategories of disease progression exist within established histological grades of articular cartilage (AC). Methods: Based on H&E and safranin-O staining of AC sections obtained from 18 knee arthroplasty surgeries, 30 samples ranging from Mankin Scoring System grade 1 through 5 were identified. Immunohistochemical (IHC) analysis for collagen type II and aggrecan was performed on serial sections of the paraffin-embedded AC samples. Six AC samples from each of the five Mankin Scoring System grades were examined. Results: Significant IHC differences in collagen type II and aggrecan deposition were seen within AC samples from all five histological grades. The range of IHC differences in collagen type II and aggrecan increased with increasing histological grade. A change in the pattern of collagen type II deposition was observed in MG-3 AC that was consistent with a switch in collagen type II metabolism. Conclusions: IHC staining of collagen type II and aggrecan can identify differences within histological grades of AC that are consistent with the existence of molecular subcategories. These differences were detectable even within the lowest histological grades; therefore the use of IHC staining can further enhance and refine the scoring of AC deterioration in early osteoarthritis (OA). Furthermore, the changes seen in the deposition pattern for both aggrecan and collagen type II suggest that they could be used to monitor key molecular events in OA progression. These findings also underscore the need for the development of IHC scoring criteria.