Effects of walking combined with restricted leg blood flow on mTOR and MAPK signaling in young men
Effects of walking combined with restricted leg blood flow on mTOR and MAPK signaling in young men
复制标题
步行结合限制腿部血流对年轻男性 mTOR 和 MAPK 信号传导的影响
DOI：
10.1111/apha.12243
复制
发表时间：
2014
影响因子：
6.3
通讯作者：
Naito H
中科院分区：
文献类型：
--
作者：
Ozaki H;Kakigi R;Kobayashi H;Loenneke JP;Abe T;Naito H
步行与血流量减少相结合（BFR-步行）会引起肌肉肥大。然而，这种反应背后的骨骼肌细胞内信号传导目前尚不清楚。目的是研究 BFR-walk 对年轻男性雷帕霉素靶点 (mTOR) 和丝裂原激活蛋白激酶 (MAPK) 信号通路的影响。方法六名年轻男性以预定最大摄氧量的 55% 在跑步机上行走 20 分钟。仅在一条腿（BFR-Leg，外部压力为 240 mmHg）的最近端大腿上施加压力袖带，而另一条腿（CON-Leg）在行走时没有 BFR。运动前和运动后 3 h 双腿股外侧肌进行肌肉活检。结果运动后双腿 Erk1/2 磷酸化水平显着升高（P<0.05）；运动后 Erk1/2 磷酸化水平显着升高（P<0.05）；然而，只有 BFR-Leg 的 p38 磷酸化增加。对于 mTOR 信号传导，步行前后 Akt、mTOR 或 S6K1 磷酸化水平没有变化。然而，与 CON-Leg 相比，步行 3 小时后 BFR-Leg 的 eEF2 磷酸化水平显着降低 (P<0.05)。结论 BFR-步行运动可能激活一些与年轻男性肌肉肥大相关的细胞内信号级联。
Walking combined with blood flow reduction (BFR‐walk) elicits muscle hypertrophy. However, the skeletal muscle intracellular signalling behind this response is currently unknown.AimTo investigate the effects of BFR‐walk on mechanistic target of rapamycin (mTOR) and mitogen‐activated protein kinase (MAPK) signalling pathways in young men.MethodsSix young men performed 20 min of treadmill walking at 55% of their predetermined maximum oxygen uptake. A pressure cuff belt was applied to the most proximal thigh of only one leg (BFR‐Leg, external compression was 240 mmHg), whereas the other leg (CON‐Leg) was without BFR during walking. Muscle biopsies were taken from the vastus lateralis of the CON‐Leg before exercise and in both legs 3 h after exercise.ResultsErk1/2 phosphorylation levels were significantly (P< 0.05) increased after exercise in both legs; however, only the BFR‐Leg saw an increased phosphorylation of p38. For mTOR signalling, there were no changes in Akt, mTOR or S6K1 phosphorylation levels before or after walking. However, eEF2 phosphorylation level was significantly (P< 0.05) lower for the BFR‐Leg 3 h after walking compared with CON‐Leg.ConclusionBFR‐walk exercise may activate some intracellular signalling cascades that are associated with muscle hypertrophy in young men.