Gelsolin concentration in cerebrospinal fluid from patients with multiple sclerosis and other neurological disorders
Gelsolin concentration in cerebrospinal fluid from patients with multiple sclerosis and other neurological disorders
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DOI：
10.1111/j.1468-1331.2008.02133.x
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发表时间：
2008-06-01
影响因子：
5.1
通讯作者：
Janmey, P. A.
中科院分区：
文献类型：
--
作者：
Kulakowska, A.;Drozdowski, W.;Janmey, P. A.
背景资料：凝溶胶蛋白是一种高度保守的细胞内肌动蛋白结合蛋白，具有细胞外同种型，血浆凝溶胶蛋白，其功能尚未明确定义。材料与方法：在这项研究中，我们确定了凝溶胶蛋白浓度的血液和脑脊液（CSF）从25名受试者使用免疫印迹和功能测定，定量凝溶胶蛋白的能力，加速肌动蛋白聚合。结果：通过定量免疫印迹法测定的CSF中的凝溶胶蛋白浓度为1.2-15.9 μ g/ml（平均5.9 +/- 3.8 μ g/ml）。在从诊断患有不改变标准CSF临床试验的病症的患者[（特发性头痛、椎间盘病引起的坐骨痛和特发性（贝尔氏）面神经麻痹或压迫性桡神经病变）]中获得的样品中，平均凝溶胶蛋白浓度为7.2 +/- 4.3 μ g/ml。相比之下，从诊断为多发性硬化的患者获得的样品中的凝溶胶蛋白浓度为2.1 ± 0.7 μ g/ml，并且在从蛛网膜下腔出血恢复的患者中发现了类似的低浓度。通过肌动蛋白聚合试验测定的CSF凝溶胶蛋白浓度范围为0.61-9.97 μ g/ml（平均3.6 +/- 2.2 μ g/ml）。这些较低的值相比，从免疫印迹分析表明，CSF凝溶胶蛋白可能会结合其他CSF分子，导致其肌动蛋白结合活性的降低。结论：这里呈现的结果表明，CSF凝溶胶蛋白浓度在某些神经系统疾病中显著改变，包括多发性硬化，表明CSF凝溶胶蛋白水平用于诊断目的的可能效用。
Background: Gelsolin is a highly conserved intracellular actin-binding protein with an extracellular isoform, plasma gelsolin, for which there is not yet a clearly defined function. Materials and methods: In this study, we determined gelsolin concentrations in blood and cerebrospinal fluid (CSF) obtained from 25 subjects using immuno-blotting and a functional assay that quantifies gelsolin's ability to accelerate actin polymerization. Results: The gelsolin concentration in CSF, determined by quantitative immunoblotting was 1.2-15.9 mu g/ml (average 5.9 +/- 3.8 mu g/ml). In samples obtained from patients diagnosed with conditions that do not alter standard CSF clinical tests [(idiopathic cephalgia, ischialgia due to discopathy, and idiopathic (Bell's) facial nerve palsy or entrapment radial neuropathy)], the average gelsolin concentration was 7.2 +/- 4.3 mu g/ml. In contrast, the gelsolin concentration in samples obtained from patients diagnosed with multiple sclerosis was 2.1 +/- 0.7 mu g/ml, and a similar low concentration was found in a patient recovering from a subarachnoid hemorrhage. The range of CSF gelsolin concentrations determined by the actin polymerization assay was 0.61-9.97 mu g/ml (average 3.6 +/- 2.2 mu g/ml). These lower values compared with those obtained from immunoblotting analysis suggest that CSF gelsolin may bind other CSF molecules leading to a reduction of its actin-binding activity. Conclusions: The results presented here show that CSF gelsolin concentration is significantly altered in certain neurological conditions, including multiple sclerosis, indicating the possible utility of CSF gelsolin levels for diagnostic purposes.