The cytochrome system of heterotrophically-grown Rhodopseudomonas spheroides.
The cytochrome system of heterotrophically-grown Rhodopseudomonas spheroides.
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异养生长的球状红假单胞菌的细胞色素系统。
DOI：
10.1016/0005-2728(70)90139-8
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
O. Jones
中科院分区：
文献类型：
--
作者：
F. R. Whale;O. Jones
在黑暗条件下，对红球藻球形体进行有氧连续培养，得到了细菌叶绿素含量较低的细胞。细胞破碎后得到的上清液中含有细胞色素的混合物，显然是这种类型的细胞色素;这些细胞色素在77°K下用光谱法分解为三种成分。在77°K还原-氧化差谱中，α、β和γ带的位置分别为547、520和417 nm，549、520和420 nm和551、521和421 nm。547 nm的细胞色素不易被抗坏血酸/四甲基对苯二胺（TMPD）还原，551 nm的细胞色素是可自氧化的。547-和549-nm的细胞色素被发现在颗粒部分，都减少了NADH或琥珀酸在有氧稳定状态，并成为更多的氧化在抗霉素或2-庚基-4-羟基醌-N-氧化物（HQNO）的存在下。可溶性组分的CO差光谱表明，它也含有细胞色素，在569，539和416 nm处获得最大值。还原的微量氧化谱在607和445 nm处有谱带，CO差谱在445 nm处有一个谷，表明存在同型末端氧化酶。与CO反应较慢的组分在540和570 nm处产生最大值，在428 nm处产生波谷，有时在416 nm处产生最大值。这些可能是由于颗粒中CO结合颜料的混合物。b型细胞色素的低温差光谱中α峰不对称。两种组分被光谱分离;主要组分是底物还原的，在560 nm（77°K时为557 nm）处吸收;第二种组分在565 nm（77°K时为562 nm）处吸收，在连二亚硫酸盐还原-底物还原光谱中发现。在抗霉素存在下，光照引起c-氧化和b-型组分的还原。
Rhodopseudomonas spheroideswas grown aerobically in the dark in continuous culture yielding cells with a low bacteriochlorophyll content. The supernatant obtained after cell breakage contained a mixture of cytochromes, apparently of thec-type; these were resolved by spectroscopy at 77°K into three components. The positions of the α, β and γ bands in reducedminusoxidised difference spectra at 77°K were 547, 520 and 417 nm; 549, 520 and 420 nm and 551, 521 and 421 nm, respectively. The 547-nm cytochrome was not readily reduced by ascorbate/tetramethyl-p-phenylene diamine (TMPD) and the 551-nm cytochrome was autoxidisable. Both the 547- and the 549-nm cytochrome were found in the particulate fraction and both were reduced by NADH or succinate in the aerobic steady state and became more oxidised in the presence of antimycin or 2-heptyl-4-hydroxyquinone-N-oxide (HQNO). CO difference spectra of the soluble fraction indicated that it also contained cytochromoidc; maxima were obtained at 569, 539 and 416 nm.The particles were found to contain a mixture of cytochromes. Reducedminusoxidised spectra showed bands at 607 and 445 nm and there was a trough in CO difference spectra at 445 nm indicating the presence of anatype terminal oxidase. Components reacting more slowly with CO yielded maxima at 540 and 570 nm with a trough at 428 nm and sometimes a maximum at 416 nm. These may be due to a mixture of CO-binding pigments in the particles. The α-peak of theb-type cytochromes was asymmetrical in low-temperature difference spectra. Two components were separated spectroscopically; the main component was substrate-reducible and absorbed at 560 nm (557 nm at 77°K); a second component absorbed at 565 nm (562 nm at 77°K) and was found in dithionite-reducedminussubstrate-reduced spectra.The small amount of bacterial chlorophyll present in the particles was photo-synthetically active. In the presence of antimycin illumination causedc-oxidation and the reduction of bothb-type components.