Prebiotic effect of fructo-oligosaccharides on the inner ear of DBA/2 J mice with early-onset progressive hearing loss
Prebiotic effect of fructo-oligosaccharides on the inner ear of DBA/2 J mice with early-onset progressive hearing loss
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DOI：
10.1016/j.jnutbio.2019.108247
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发表时间：
2020-01-01
影响因子：
5.6
通讯作者：
Tanabe, Kenichi
中科院分区：
文献类型：
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作者：
Kondo, Takako;Saigo, Saori;Tanabe, Kenichi
营养和饮食习惯有助于感音神经性听力损失（SNHL）的发生和发展。低聚果糖（FOS）是一种不易消化的低聚糖，被称为益生元，可增强短链脂肪酸（SCFA）的产生和抗氧化活性。虽然大量的研究表明，FOS作为益生元在预防与生活方式有关的疾病方面发挥作用，但对内耳的影响知之甚少。本研究旨在探讨FOS对早发性进行性聋DBA/2 J小鼠内耳螺旋神经节神经元（SGN）基因表达的影响及其对SGN的保护作用。DBA/2 J小鼠饲喂对照饲料或含10%（w/w）FOS的FOS饲料8周。对喂食FOS饮食的小鼠的分析揭示了肠道植物群的变化，包括拟杆菌门和厚壁菌门的比例倒置，随后是盲肠中SCFA的显著增加和血清中氧化应激标志物的减少。在内耳，神经营养因子，脑源性神经营养因子（BDNF），其受体，酪氨酸激酶受体B（Trkb），和SCFA受体，游离脂肪酸受体3（FFAR 3）的基因表达，FOS增加。此外，在福斯喂养的小鼠中，内耳中SGN的存活率得以维持。总之，这些结果表明，由于益生元效应导致的肠道植物群的组成变化可能参与了SNHL（C）2019 Elsevier Inc.的进展。All rights reserved.
Nutrition and dietary habits contribute to the onset and progression of sensorineural hearing loss (SNHL). Fructo-oligosaccharides (FOS) are non-digestible oligosaccharides and are known as prebiotics, which enhance short-chain fatty acid (SCFA) production and antioxidant activity. Although a substantial number of studies have shown that FOS play a role in the prevention of lifestyle-related diseases as prebiotics, little is known about the effects on the inner ear. The purpose of this study is to investigate the effect of FOS on gene expression and spiral ganglion neuron (SGN) protection in the inner ear of DBA/2 J mice, which is a model for early-onset progressive hearing loss. DBA/2 J mice were fed either control diet or FOS diet contained 10% (w/w) of FOS for 8 weeks. Analysis of mice fed the FOS diet revealed a change in intestinal flora including an inversion of the ratio of Bacteroidetes and Firmicutes, which was followed by a significant increase in SCFAs in the cecum and a decrease in an oxidative stress marker in the serum. In the inner ear, gene expression of neurotrophin, brain-derived neurotrophic factor (BDNF), its receptor, tyrosine kinase receptor b (Trkb), and the SCFA receptor, free fatty acid receptor 3 (FFAR3), were increased by FOS. In addition, the survival rate of SGNs in the inner ear was maintained in FOS-fed mice. Altogether, these results suggest that a compositional variation of the intestinal flora due to a prebiotic effect may be involved in the progression of SNHL (C) 2019 Elsevier Inc. All rights reserved.