PprA:: a protein implicated in radioresistance of Deinococcus radiodurans stimulates catalase activity in Escherichia coli
PprA:: a protein implicated in radioresistance of Deinococcus radiodurans stimulates catalase activity in Escherichia coli
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DOI：
10.1007/s00253-006-0340-7
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发表时间：
2006-10-01
影响因子：
5
通讯作者：
Misra, Hari S.
中科院分区：
文献类型：
--
作者：
Kota, Swathi;Misra, Hari S.
PPRA：一种促进DNA修复的多效性蛋白，在耐辐射葡萄球菌的辐射抗性中发挥作用。在本研究中，我们研究了辐射和氧化应激对表达PPRA的转基因大肠杆菌的影响。从耐辐射葡萄球菌KR1中克隆了pprA基因，并在大肠杆菌中进行了表达。与对照相比，表达PPRA的转基因大肠杆菌细胞对过氧化氢的耐受性提高了两到三倍。与非重组对照相比，凯特对体内过氧化氢酶活性的刺激作用是非重组对照的2.8倍。此外，纯化的PPRA对大肠杆菌过氧化氢酶在溶液中的活性有1.7倍的刺激作用。在体内和体外观察到的PPRA对过氧化氢酶活性的影响在PPRA抗体存在的情况下恢复到正常水平。这些结果表明，在表达PPRA的大肠杆菌中，氧化应激耐受性的增强是由于PPRA刺激过氧化氢酶活性，可能是通过这些蛋白的相互作用。
PprA: a pleiotropic protein promoting DNA repair, role in radiation resistance of Deinococcus radiodurans was demonstrated. In this study, the effect of radiation and oxidative stress on transgenic Escherichia coli expressing pprA has been studied. The pprA gene from D. radiodurans KR1 was cloned and expressed in E. coli. Transgenic E. coli cells expressing PprA showed twofold to threefold higher tolerance to hydrogen peroxide as compared to control. The 2.8-fold in vivo stimulation of catalase activity largely contributed by KatE was observed as compared to nonrecombinant control. Furthermore, the purified PprA could stimulate the E. coli catalase activity by 1.7-fold in solution. The effect of PprA on catalase activity observed both in vivo and in vitro was reverted to normal levels in the presence of PprA antibodies. The results suggest that enhanced oxidative stress tolerance in E. coli expressing PprA was due to the PprA stimulation of catalase activity, perhaps through the interaction of these proteins.