Syngeneic mouse mammary carcinoma cell lines: Two closely related cell lines with divergent metastatic behavior
Syngeneic mouse mammary carcinoma cell lines: Two closely related cell lines with divergent metastatic behavior
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DOI：
10.1007/s10585-005-2908-5
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Gregg, JP
中科院分区：
文献类型：
--
作者：
Borowsky, AD;Namba, R;Gregg, JP
从FVB/N- tg （MMTV-PyVmT）和FVB/N- tg （MMTV-PyVmT(Y315F/Y322F)）小鼠乳腺癌中分离出两种具有不同转移潜能的细胞系Met-1（fvb2）和DB-7(fvb2)，将其移植到同基因的FVB/N宿主体内并进行表征。经过多轮体外培养和体内移植，这些细胞系保持了稳定的形态和生物学表型。来源于FVB/N- tg （MMTV-PyVmT）肿瘤的Met-1（fvb2）细胞系在移植到女性FVB/N乳腺脂肪垫时表现出侵袭性生长和100%转移。在Y315F/Y322F位点有“双碱基”修饰的FVB/N-Tg（MMTV-PyVmT(Y315F/Y322F)）衍生的DB-7（fvb2）细胞系在移植到乳腺脂肪垫时生长更快，但转移率较低（17%）。Met1（fvb2）细胞显示AKT的高激活，而DB-7（fvb2）细胞显示AKT的低激活。两种细胞系的DNA含量和基因表达水平在多代中都是稳定的。因此，这两种细胞系为研究转移调节剂、AKT分子通路相互作用以及发现基因靶点和标记物提供了稳定、可重复的资源。
Two cell lines, Met-1(fvb2) and DB-7(fvb2), with different metastatic potential, were derived from mammary carcinomas in FVB/N-Tg(MMTV-PyVmT) and FVB/N-Tg(MMTV-PyVmT(Y315F/Y322F)) mice, transplanted into syngeneic FVB/N hosts and characterized. The lines maintain a stable morphological and biological phenotype after multiple rounds of in vitro culture and in vivo transplantation. The Met-1(fvb2) line derived from a FVB/N-Tg(MMTV-PyVmT) tumor exhibits invasive growth and 100% metastases when transplanted into the females FVB/N mammary fat pad. The DB-7(fvb2) line derived from the FVB/N-Tg(MMTV-PyVmT(Y315F/Y322F)) with a "double base" modification at Y315F/Y322F exhibits more rapid growth when transplanted into the mammary fat pad, but a lower rate of metastasis (17%). The Met1(fvb2) cells show high activation of AKT, while DB-7(fvb2) cells show very low levels of AKT activation. The DNA content and gene expression levels of both cell lines are stable over multiple generations. Therefore, these two cell lines provide a stable, reproducible resource for the study of metastasis modulators, AKT molecular pathway interactions, and gene target and marker discovery.