Immunologic functions of isolated human lymphocyte subpopulations. I. Quantitative isolation of human T and B cells and response to mitogens.
Immunologic functions of isolated human lymphocyte subpopulations. I. Quantitative isolation of human T and B cells and response to mitogens.
复制标题
分离的人淋巴细胞亚群的免疫功能。
作者：
L. Chess;R. Macdermott;S. Schlossman
采用Sephadex G-200免疫吸收柱结合兔抗人Fab分离人外周血淋巴细胞。定量地恢复了两个不同的细胞群。通过免疫荧光和E染色特性判断，直接通过柱而不结合的细胞是T细胞。另一方面，保留的能被人Ig特异性定量洗脱的细胞为τ；97%的B细胞，根据荧光和结瘤标准判断。T细胞和B细胞在植物血凝素、豆豆蛋白A和美洲陆有丝分裂原的作用下均显著结合3 h -胸腺嘧啶，而在内毒素或抗人Fab的作用下均未发生增殖。有丝分裂原诱导的胚细胞在T细胞和B细胞培养中保持了细胞表面特征。T细胞原细胞表面Ig缺失；90%的B细胞原细胞表面含有Ig。
A Sephadex G-200 immunoabsorbent column bound with pure rabbit antihuman Fab was used to fractionate human peripheral lymphocytes. Two distinct populations of cells were quantitatively recovered. The cells passing directly through the column without binding were T cells, as judged by both immunofluorescent and E rosetting properties. On the other hand, the retained cells which could be specifically and quantitatively eluted with human Ig were τ;97% B cells, as judged by the fluorescence and rosetting criteria. Both T and B cells incorporated 3 H-thymidine significantly in response to phytohemagglutinin, concanavalin A, and pokeweed mitogen, while neither population proliferated in response to endotoxin or antihuman Fab. The blast cells induced by mitogen in T or B cell cultures retained their cell surface characteristics. T cell blasts were devoid of surface Ig while τ;90% B cell blasts had Ig on their cell surface.