Cdc2p controls the forkhead transcription factor Fkh2p by phosphorylation during sexual differentiation in fission yeast
Cdc2p controls the forkhead transcription factor Fkh2p by phosphorylation during sexual differentiation in fission yeast
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DOI：
10.1038/sj.emboj.7601949
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发表时间：
2008-01
期刊：
影响因子：
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通讯作者：
M. Shimada;C. Yamada‐Namikawa;Yuko Murakami-Tonami;Takashi Yoshida;M. Nakanishi;T. Urano;H. Murakami
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文献类型：
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作者：
M. Shimada;C. Yamada‐Namikawa;Yuko Murakami-Tonami;Takashi Yoshida;M. Nakanishi;T. Urano;H. Murakami
在大多数真核生物中，细胞周期蛋白依赖性激酶（Cdks）在控制细胞周期进程中发挥核心作用。Cdks在有丝分裂结束到下一个细胞周期开始以及性分化期间失活。叉头型转录因子Fkh2p是裂殖酵母裂殖酵母中许多基因的周期性表达和有效交配所必需的。然而，负责协调细胞周期进程与性分化的机制仍然未知。我们现在表明，Fkh2p被Cdc2p（Cdk 1）磷酸化，并且通过这种Cdk在T314或S462上磷酸化Fkh2p，通过阻止ste11+转录的诱导来阻断粟酒裂殖酵母中的交配，而ste11+转录是性发育开始所必需的。我们认为Cdks和叉头转录因子之间的功能相互作用可能与细胞有丝分裂周期和性分化有关。
In most eukaryotes, cyclin‐dependent kinases (Cdks) play a central role in control of cell‐cycle progression. Cdks are inactivated from the end of mitosis to the start of the next cell cycle as well as during sexual differentiation. The forkhead‐type transcription factor Fkh2p is required for the periodic expression of many genes and for efficient mating in the fission yeastSchizosaccharomyces pombe. However, the mechanism responsible for coordination of cell‐cycle progression with sexual differentiation is still unknown. We now show that Fkh2p is phosphorylated by Cdc2p (Cdk1) and that phosphorylation of Fkh2p on T314 or S462 by this Cdk blocks mating inS. pombeby preventing the induction ofste11+transcription, which is required for the onset of sexual development. We propose that functional interaction between Cdks and forkhead transcription factors may link the mitotic cell cycle and sexual differentiation.