Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes.
Partially Protective Immunity Induced by a 20 kDa Protein Secreted by Trichinella spiralis Stichocytes.
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旋毛虫粘细胞分泌的 20 kDa 蛋白质诱导的部分保护性免疫
DOI：
10.1371/journal.pone.0136189
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhu X
中科院分区：
文献类型：
--
作者：
Bi K;Yang J;Wang L;Gu Y;Zhan B;Zhu X
背景旋毛虫感染在动物模型中诱导针对再感染的保护性免疫。旋毛虫感染过程中获得性免疫的抗原的鉴定对旋毛虫感染的疫苗研制和免疫诊断具有重要意义。方法和结果T.用20,000株感染性旋毛虫的猪血清对旋毛虫成虫cDNA文库进行免疫筛选。螺旋幼虫共鉴定出43个阳性克隆，编码28种蛋白，其中一种为20 kDa的Ts-ES-1，存在于旋毛虫的排泄/分泌（ES）产物中。旋毛虫成虫和肌蠕虫。TS-ES-1由172个氨基酸组成，前20个氨基酸含有典型的信号肽。Ts-ES-1基因在成体和肌幼虫期均有表达。用ISA 50 v2佐剂配制的重组Ts-ES-1（rTs-ES-1）免疫小鼠，在T.与佐剂对照组相比（p<0.01）。rTs-ES-1诱导的保护作用与高水平的特异性抗Ts-ES-1 IgG抗体和Th 1/Th 2混合免疫应答相关。结论新鉴定的rTs-ES-1蛋白是旋毛虫自然感染时毛细胞分泌的一种免疫优势蛋白，可诱导旋毛虫免疫小鼠产生部分保护性免疫。因此，rTs-ES-1是一个潜在的候选旋毛虫病疫苗的开发。
Background Trichinella spiralis infection induces protective immunity against re-infection in animal models. Identification of the antigens eliciting acquired immunity during infection is important for vaccine development against Trichinella infection and immunodiagnosis. Methods and Findings The T. spiralis adult cDNA library was immunoscreened with sera from pigs experimentally infected with 20,000 infective T. spiralis larvae. Total 43 positive clones encoding for 28 proteins were identified; one of the immunodominant proteins was 20 kDa Ts-ES-1 secreted by Trichinella stichocytes and existing in the excretory/secretory (ES) products of T. spiralis adult and muscle larval worms. Ts-ES-1 contains 172 amino acids with a typical signal peptide in the first 20 amino acids. The expression of Ts-ES-1 was detected in both the adult and muscle larval stages at the mRNA and protein expression levels. Mice immunized with recombinant Ts-ES-1 (rTs-ES-1) formulated with ISA50v2 adjuvant exhibited a significant worm reduction in both the adult worm (27%) and muscle larvae burden (42.1%) after a challenge with T. spiralis compared to the adjuvant control group (p<0.01). The rTs-ES-1-induced protection was associated with a high level of specific anti-Ts-ES-1 IgG antibodies and a Th1/Th2 mixed immune response. Conclusion The newly identified rTs-ES-1 is an immunodominant protein secreted by Trichinella stichocytes during natural infection and enables to the induction of partial protective immunity in vaccinated mice against Trichinella infection. Therefore, rTs-ES-1 is a potential candidate for vaccine development against trichinellosis.