SEQUESTERING OF IMMUNOGLOBULINS BY ASTROCYTES AFTER CORTICAL LESION AND HOMOGRAFTING OF FETAL CORTEX
SEQUESTERING OF IMMUNOGLOBULINS BY ASTROCYTES AFTER CORTICAL LESION AND HOMOGRAFTING OF FETAL CORTEX
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DOI：
10.1016/0736-5748(93)90072-l
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发表时间：
1993-04-01
影响因子：
1.8
通讯作者：
GOLDBERG, WJ
中科院分区：
文献类型：
--
作者：
BERNSTEIN, JJ;WILLINGHAM, LA;GOLDBERG, WJ
本文研究了成年大鼠大脑皮质损伤后和胎鼠大脑皮质移植后正常、反应性和迁移性星形胶质细胞免疫球蛋白（IgG、IgM、伊加）含量的变化。在成年Sprague-Dawley大鼠的躯体运动皮质（前囟下）中抽吸植入囊袋。将在植物凝集素Phaseolus vulgaris leucoagglutinin（移植物预标记物）中孵育的胎鼠（E14）半皮质置于抽吸袋或空袋（对照）中。术后3-60天，对脑切片进行免疫球蛋白-GFAP或菜豆白细胞凝集素-GFAP的化学双标记。原位成熟或胎儿星形胶质细胞不含免疫球蛋白。在仅囊袋动物中，囊袋内衬和囊袋下的单个反应性星形胶质细胞对大鼠IgG、IgM和伊加呈阳性（3-60天）。在移植动物中，移植物衍生的星形胶质细胞（菜豆白细胞凝集素-GFAP阳性）与宿主反应性星形胶质细胞（菜豆白细胞凝集素阴性）混合，衬在口袋里。两类星形胶质细胞均含有免疫球蛋白IgG、IgM和伊加。免疫球蛋白阳性移植物来源的星形胶质细胞迁移到胼胝体、扣带回和缰核。这些结果表明，星形胶质细胞隔离免疫球蛋白和可能的其他血清蛋白作为一个关键的功能，在恢复稳态损伤或患病的神经系统。
The immunoglobulin (IgG, IgM, IgA) content of normal, reactive and migrated rat astrocytes was studied in lesioned adult rat cortex and after fetal cortex grafts. Implantation pockets were aspirated in the somatomotor cortex (under bregma) of adult Sprague-Dawley rats. A fetal (E14) rat hemicortex incubated in the plant lectin Phaseolus vulgaris leucoagglutinin (graft premarker) was placed in the aspiration pocket or the pocket left empty (control). Sections of brain were immunohistochemically double labeled for immunoglobulins-GFAP or Phaseolus vulgaris leucoagglutinin-GFAP 3-60 days postoperative. Mature or fetal astrocytes in situ did not contain immunoglobulins. In pocket-only animals, individual reactive astrocytes lining and subjacent to the pocket were positive for rat IgG, IgM and IgA (3-60 days). In grafted animals, graft derived astrocytes (Phaseolus vulgaris leucoagglutinin-GFAP positive) were intermingled with host reactive astrocytes (Phaseolus vulgaris leucoagglutinin negative) lining and subjacent to the pocket. Both classes of astrocytes contained immunoglobulins IgG, IgM and IgA. Immunoglobulin positive graft derived astrocytes migrated into the corpus callosum, cingulum and habenula. These results demonstrate that astrocytes sequester immunoglobulins and probably other serum proteins as a critical function in restoring homeostasis to the injured or diseased nervous system.