MicroRNA-146a regulates the maturation process and pro-inflammatory cytokine secretion by targeting CD40L in oxLDL-stimulated dendritic cells
MicroRNA-146a regulates the maturation process and pro-inflammatory cytokine secretion by targeting CD40L in oxLDL-stimulated dendritic cells
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DOI：
10.1016/j.febslet.2011.01.010
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发表时间：
2011-02-04
期刊：
影响因子：
3.5
通讯作者：
Zhu, Jianhua
中科院分区：
文献类型：
--
作者：
Chen, Ting;Li, Zhoubin;Zhu, Jianhua
越来越多的证据表明microRNAs（miRNAs）在伴随炎症反应的细胞增殖、凋亡和分化中发挥重要作用。然而，miRNAs是否与树突状细胞（DC）对氧化低密度脂蛋白（oxLDL）刺激的免疫炎症反应有关尚不清楚。本研究旨在探讨miRNA与脂质过负荷的关系以及动脉粥样硬化的免疫炎症机制。用miR-146 a模拟物和抑制剂转染人原代单核细胞来源的DC，然后用oxLDL刺激。对于DC免疫表型的流式细胞术分析，收集上清液以确定炎性趋化因子标志物。我们的研究清楚地揭示了miRNA-146 a通过靶向ox-LDL刺激的DC中的CD 40 L来调节DC中的成熟过程和促炎细胞因子分泌。(C)2011年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
There is increasing evidence that microRNAs (miRNAs) play important roles in cell proliferation, apoptosis and differentiation that accompany inflammatory responses. However, whether miRNAs are associated with dendritic cell (DC) immuno-inflammatory responses to oxidized low density lipoprotein (oxLDL) stimulation is yet unknown. Our study aims to explore the link of miRNA to lipid-overload and the immuno-inflammatory mechanism for atherosclerosis. Human primary monocyte-derived DCs were transfected with miR-146a mimics and inhibitor, and then stimulated by oxLDL. For the flow cytometric analysis of the DC immunophenotype, supernatants were collected to determine inflammatory chemokine markers. Our study clearly revealed that miRNA-146a regulates the maturation process and pro-inflammatory cytokine secretion in DCs by targeting CD40L in ox-LDL-stimulated DCs. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.