Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats
Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats
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DOI：
10.1159/000445561
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发表时间：
2016-01-01
影响因子：
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通讯作者：
Wu, Guangwei
中科院分区：
文献类型：
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作者：
Chen, Chunxia;Chen, Wan;Wu, Guangwei
背景/目的：在本研究中，我们研究了高压氧(HBO)和地尔硫卓联合治疗对大鼠心肌缺血再灌注损伤(MIRI)模型的心脏保护作用。方法：健康SD大鼠60只，随机分为假手术组、IR组、地尔硫卓(5 mg/kg)组、高压氧(0.25 Mpa，60min)组和高压氧+地尔硫卓联合治疗组。结扎左前降支30min，再灌流60min，建立MIRI模型。结果：高压氧和地尔硫卓可显著改善心功能和心肌梗死面积，提高一氧化氮、内皮型一氧化氮合酶和三磷酸腺苷酶(Na+-K+-ATPase和Ca2+-Mg2+-ATPase)活性，降低氧应激、心肌酶和内皮素-1水平。值得注意的是，高压氧和地尔硫卓预适应显著增加了Bcl2蛋白的表达，降低了Bax蛋白和caspase3mRNA的表达。结论：联合治疗可减轻氧应激损伤，纠正能量代谢，改善内皮功能，抑制细胞凋亡，对心脏MIRI具有保护作用。(C)2016年作者(S)，S.Karger AG，巴塞尔出版
Background/Aims: In this study, we examined whether the combination of hyperbaric oxygen (HBO) and diltiazem therapy provided a cardioprotective effect on myocardial ischemia-reperfusion injury (MIRI) rat model. Methods: Sixty healthy Sprague-Dawley rats were randomly divided into sham, IR, diltiazem (5 mg/kg), HBO (0.25 MPa, 60 min) and combination therapy (HBO plus diltiazem) groups. MIRI model was established by ligating the left anterior descending for 30 min, followed by 60 min of reperfusion. Results: The results show that HBO and diltiazem preconditioning significantly improves cardiac function and myocardial infarction area, increases nitric oxide, endothelial nitric oxide synthase and ATPase (Na+-K+-ATPase and Ca2+-Mg2+-ATPase) activity and decreases levels of oxygen stress, myocardial enzymes and endothelin-1. Notably, HBO and diltiazem preconditioning significantly increased Bcl-2 protein expression and decreased Bax protein and caspase 3 mRNA expression. Conclusions: These data indicate that combination therapy protected against heart MIRI by reducing oxygen stress damage, correcting energy metabolism, improving endothelial function and inhibiting cell apoptosis. (C) 2016 The Author(s) Published by S. Karger AG, Basel