Utilization of a Protein "Shuttle" To Load Vault Nanocapsules with Gold Probes and Proteins
Utilization of a Protein "Shuttle" To Load Vault Nanocapsules with Gold Probes and Proteins
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DOI：
10.1021/nn900555d
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发表时间：
2009-10-01
期刊：
影响因子：
17.1
通讯作者：
Monbouquette, Harold G.
中科院分区：
文献类型：
--
作者：
Goldsmith, Lisa E.;Pupols, Melody;Monbouquette, Harold G.
穹窿是大的蛋白质纳米胶囊，由于其在人体中的正常存在，其大的内部体积，其由一种蛋白质的多个拷贝组成的简单结构组成，以及还提供了定制其结构的方法的重组生产系统，其可以用作药物递送载体。然而，为了使拱顶在这种应用中有效，必须证明装载胶囊内部的有效手段。在这里，我们描述了使用一个域的拱顶内腔相关蛋白作为载体，以靶向金纳米簇和异源His标记的蛋白质的拱顶内壁上的特定结合位点。
Vaults are large protein nanocapsules that may be useful as drug delivery vehicles due to their normal presence in humans, their large interior volume, their simple structural composition consisting of multiple copies of one protein, and a recombinant production system that also provides a means to tailor their structure. However, for vaults to be effective in such applications, efficient means to load the interiors of the capsules must be demonstrated. Here we describe the use of a domain derived from a vault lumen-associated protein as a carrier to target both gold nanoclusters and heterologous His-tagged proteins to specific binding sites on the vault interior wall.