Comparative analysis of erythrocyte hemolysis, plasma parameters and metabolic features in red crucian carp (Carassius auratus red var) and triploid hybrid fish following Aeromonas hydrophila challenge
Comparative analysis of erythrocyte hemolysis, plasma parameters and metabolic features in red crucian carp (Carassius auratus red var) and triploid hybrid fish following Aeromonas hydrophila challenge
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嗜水气单胞菌攻击后红鲫鱼和三倍体杂交鱼红细胞溶血、血浆参数和代谢特征的比较分析
DOI：
10.1016/j.fsi.2021.09.025
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发表时间：
2021
影响因子：
4.7
通讯作者：
Qing-Feng Liu
中科院分区：
文献类型：
--
作者：
Ning-Xia Xiong;Sheng-Wei Luo;Lan-Fen Fan;Zhuang-Wen Mao;Kai-Kun Luo;Shao-Jun Liu;Chang Wu;Fang-Zhou Hu;Shi Wang;Ming Wen;Qing-Feng Liu
嗜水气单胞菌对淡水鱼的生存构成极大威胁。在本研究中，A。2 N = 100的红鲫鱼（RCC）和三倍体杂交鱼（3 N = 150）的总抗氧化能力（T-AOC）、总超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、碱性磷酸酶（ALP）和溶菌酶（LZM）水平均降低。hydrophilachallenge。热休克蛋白90α (HSP90α)、基质金属蛋白酶9 （MMP-9）、游离脂肪酸受体3 （FFAR3）、对氧磷酶2 （PON2）、胞质磷脂酶A2 （cPLA2）表达水平升高。hydrophila-infected鱼。此外,一个。亲水挑战可显著增加RCC和3n中皮质醇、亮氨酸、异亮氨酸、谷氨酸和多不饱和脂肪酸（PUFAs）的表达，而糖酵解和三羧酸循环则表现为失活。我们从受a胁迫的不同倍性鲤的代谢谱中发现了差异脂肪酸衍生物及其代谢网络作为关键的生物标志物。hydrophilainfection。这些结果突出了不同倍性鲤对细菌感染的代谢策略的比较。
Aeromonas hydrophila can pose a great threat to survival of freshwater fish. In this study,A. hydrophilachallenge could promote the erythrocyte hemolysis, increase free hemoglobin (FHB) level and generate malondialdehyde (MDA) production in plasma but decrease the levels of total antioxidant capacity (T-AOC), total superoxide dismutase (SOD), catalase (CAT), alkaline phosphatase (ALP) and lysozyme (LZM) of red crucian carp (RCC, 2 N = 100) and triploid hybrid fish (3 N fish, 3 N = 150) followingA. hydrophilachallenge. Elevated expression levels of heat shock protein 90 alpha (HSP90α), matrix metalloproteinase 9 (MMP-9), free fatty acid receptor 3 (FFAR3), paraoxonase 2 (PON2) and cytosolic phospholipase A2 (cPLA2) were observed inA. hydrophila-infected fish. In addition,A. hydrophilachallenge could significantly increase expressions of cortisol, leucine, isoleucine, glutamate and polyunsaturated fatty acids (PUFAs) in RCC and 3 N, while glycolysis and tricarboxylic acid cycle appeared to be inactive. We identified differential fatty acid derivatives and their metabolic networks as crucial biomarkers from metabolic profiles of different ploidy cyprinid fish subjected toA. hydrophilainfection. These results highlighted the comparative metabolic strategy of different ploidy cyprinid fish against bacterial infection.