Transcranial imaging of somatotopic map plasticity after tail cut in mice
Transcranial imaging of somatotopic map plasticity after tail cut in mice
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DOI：
10.1016/j.brainres.2010.01.020
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发表时间：
2010-03-10
期刊：
影响因子：
2.9
通讯作者：
Shibuki, Katsuei
中科院分区：
文献类型：
--
作者：
Kitaura, Hiroki;Hishida, Ryuichi;Shibuki, Katsuei
外周传入去神经支配诱导初级体感觉皮层的体位图重组（S1）。在本研究中，我们研究了断尾后的体位图可塑性。在出生后2-3天的新生小鼠和8周龄的成年小鼠用乙醚麻醉，并从尖端切断约三分之二的尾巴。两组小鼠在10周龄时均以1.7 g/kg, i.p.的氨基甲酸乙酯麻醉，并在Si中进行经颅黄蛋白荧光成像。用振动刺激对侧后爪或尾，可引起脑内神经活动。被后爪、尾巴基部和尾巴尖端刺激激活的皮质区域按照内侧和后部的顺序排列。断尾小鼠的尾基区向与对照组小鼠的尾尖相对应的更内侧、更后的区域移动。在新生和成年断尾小鼠中均观察到尾基底区域的移动，表明在8周之前没有一个关键时期。切尾小鼠的尾基底区相对于神经鞘有内侧和后部移位。这些数据表明，经颅黄蛋白荧光成像是研究小鼠体感图可塑性的一种有用的技术。(C) 2010 Elsevier B.V.版权所有
Peripheral afferent denervation induces reorganization of somatotopic maps in the primary somatosensory cortex (S1). In the present study, we investigated somatotopic map plasticity after tail cut. Neonatal mice at postnatal days (P) 2-3 and adult mice at eight weeks of age were anesthetized with ether, and approximately two thirds of the tail was cut from the tip. Both groups of mice were anesthetized with urethane (1.7 g/kg, i.p.) at 10 weeks of age, and transcranial flavoprotein fluorescence imaging was performed in the Si. Neural activities in the Si were elicited by vibratory stimulation applied to the contralateral hindpaw or the tail in control mice. The cortical areas activated by hindpaw, tail base, and tail tip stimuli were placed in this order according to the medial and posterior direction. In mice with tail cut, the tail base area moved to the more medial and posterior area corresponding to the tail tip in control mice. The shift of the tail base area was observed in both neonatal and adult tail cut mice, indicating the absence of a critical period before eight weeks. Medial and posterior shift of the tail base area with regard to the bregma was confirmed in tail cut mice. These data suggest that transcranial flavoprotein fluorescence imaging is a useful technique for investigating somatosensory map plasticity in mice. (C) 2010 Elsevier B.V. All rights reserved.