Mechanosensing in T Lymphocyte Activation
Mechanosensing in T Lymphocyte Activation
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DOI：
10.1016/j.bpj.2011.12.011
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发表时间：
2012-01-18
影响因子：
3.4
通讯作者：
Kam, Lance C.
中科院分区：
文献类型：
--
作者：
Judokusumo, Edward;Tabdanov, Erdem;Kam, Lance C.
机械力在调节细胞功能中发挥着越来越重要的作用。该报告展示了 T 细胞使用聚丙烯酰胺凝胶呈现 CD3 和 CD28 配体的机械传感。通过 IL-2 的附着和分泌测量，初始 CD4 T 细胞表现出更强的激活，并且基质弹性模量在 10-200 kPa 范围内增加。通过将这些配体呈现在不同的表面上，该报告进一步证明机械传感与 CD3 而不是 CD28 信号传导的相关性更强。最后，Zap70 和 Src 家族激酶蛋白的磷酸特异性染色表明，底物刚性的感知至少部分是通过 T 细胞受体激活的下游过程发生的。 T 细胞对底物严格定量响应的能力为机械生物学提供了一个有趣的新模型。
Mechanical forces play an increasingly recognized role in modulating cell function. This report demonstrates mechanosensing by T cells, using polyacrylamide gels presenting ligands to CD3 and CD28. Naive CD4 T cells exhibited stronger activation, as measured by attachment and secretion of IL-2, with increasing substrate elastic modulus over the range of 10-200 kPa. By presenting these ligands on different surfaces, this report further demonstrates that mechanosensing is more strongly associated with CD3 rather than CD28 signaling. Finally, phospho-specific staining for Zap70 and Src family kinase proteins suggests that sensing of substrate rigidity occurs at least in part by processes downstream of T-cell receptor activation. The ability of T cells to quantitatively respond to substrate rigidly provides an intriguing new model for mechanobiology.