A novel HD‐Zip I/C2H2‐ZFP/WD‐repeat complex regulates the size of spine base in cucumber
A novel HD‐Zip I/C2H2‐ZFP/WD‐repeat complex regulates the size of spine base in cucumber
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新型HD-Zip I/C2H2-ZFP/WD-重复复合物调节黄瓜刺基部的大小
DOI：
10.1111/nph.17967
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发表时间：
2022
期刊：
影响因子：
9.4
通讯作者：
Luming Yang
中科院分区：
文献类型：
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作者：
Sen Yang;Yueling Wang;Huayu Zhu;Minjuan Zhang;Dengke Wang;Kuixi Xie;Pengfei Fan;Junling Dou;Dongming Liu;Bin Liu;Chunhua Chen;Yan Yan;Lijun Zhao;Luming Yang
果刺是黄瓜的重要性状，不仅影响商品品质，还影响果实的光滑度、运输和储藏。脊柱的大小由多细胞基础决定。然而，黄瓜脊柱基底调节的分子机制仍然很大程度上未知。在此，我们报告了编码 C2H2 锌指转录因子的 aspine base size1 (SBS1) 基因的基于图谱的克隆和表征。利用黄瓜近等基因系来绘制、鉴定和量化黄瓜脊碱基大小 1 (CsSBS1)。采用酵母杂交、双分子荧光互补 (BiFC)、免疫共沉淀 (Co-IP) 和 RNA 测序分析方法探讨 CsSBS1 调节刺碱基尺寸发育的分子机制。CsSBS1 在黄瓜子房中特异性表达，在果实刺中表达尤其高。 CsSBS1的过表达导致果实脊柱基部增大，而CsSBS1的RNA干扰沉默抑制了果实脊柱基部的扩张。对天然黄瓜种质的序列分析表明，CsSBS1 在小刺碱基种质中丢失，这是由于 CsSBS1 基因座中 4895 bp 片段缺失造成的。 CsSBS1可以与两个正向调节因子CsTTG1和CsGL1形成三聚体复合物，通过乙烯信号调节刺基部发育。提出了一种新型调节网络，CsGL1/CsSBS1/CsTTG1复合物在调节刺基部形成和大小方面发挥着重要作用，这为黄瓜育种者发育光滑的果实提供了策略。
Fruit spine is an important trait in cucumber, affecting not only commercial quality, but also fruit smoothness, transportation and storage. Spine size is determined by a multi‐cellular base. However, the molecular mechanism underlying the regulation of cucumber spine base remains largely unknown. Here, we report map‐based cloning and characterization of aspine base size1 (SBS1) gene, encoding a C2H2 zinc‐finger transcription factor.Near‐isogenic lines of cucumber were used to map, identify and quantify cucumberspine base size1 (CsSBS1). Yeast‐hybrid, bimolecular fluorescence complementation (BiFC), co‐immunoprecipitation (Co‐IP) and RNA‐sequencing assays were used to explore the molecular mechanism ofCsSBS1in regulating spine base size development.CsSBS1was specifically expressed in cucumber ovaries with particularly high expression in fruit spines. Overexpression ofCsSBS1resulted in large fruit spine base, while RNA‐interference silencing ofCsSBS1inhibited the expansion of fruit spine base. Sequence analysis of natural cucumber accessions revealed thatCsSBS1was lost in small spine base accessions, resulting from a 4895 bp fragment deletion inCsSBS1locus. CsSBS1 can form a trimeric complex with two positive regulators CsTTG1 and CsGL1 to regulate spine base development through ethylene signaling.A novel regulator network is proposed that the CsGL1/CsSBS1/CsTTG1 complex plays a significant role in regulating spine base formation and size, which offers a strategy for cucumber breeders to develop smooth fruit.