Accurate point-of-care serology tests for COVID-19.
Accurate point-of-care serology tests for COVID-19.
复制标题
精确的COVID-19即时血清学检测。
DOI：
10.1371/journal.pone.0248729
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Baker JR Jr
中科院分区：
文献类型：
--
作者：
Schuler CF 4th;Gherasim C;O'Shea K;Manthei DM;Chen J;Giacherio D;Troost JP;Baldwin JL;Baker JR Jr
随着新冠肺炎疫苗的问世，在医疗点(POC)环境中筛查个人先前的新冠肺炎感染和疫苗反应重新引起了人们的兴趣。我们在POC环境中前瞻性地对SARS-CoV-2尖峰和核衣壳蛋白抗体的高危个体进行筛查，以确定这是否是从既往感染中识别抗体的可行方法。对POC指棒血进行了三种EUA批准的侧向流动抗体检测，并与血清和CLIA核衣壳抗体免疫检测进行了比较。变量包括抗体类别、自聚合酶链式反应以来的时间和所使用的检测抗原。512例入选，其中104例有新冠肺炎病史，聚合酶链式反应阳性。只有三名PCR阳性的受试者需要住院，其中一人需要机械通气。POC结果与免疫分析有很好的相关性(93-97%的灵敏度)，使用血清并不能提高灵敏度或特异度。手指棒，POC新冠肺炎抗体检测在识别轻微症状受试者先前感染所产生的抗体方面非常有效。使用高复杂性血清免疫分析并没有改善筛查结果。几乎所有感染新冠肺炎的人都能产生可检测到的病毒抗体。POC抗体检测可用作新冠肺炎既往感染的筛查，并可用于评估疫苗反应。
As COVID-19 vaccines become available, screening individuals for prior COVID-19 infection and vaccine response in point-of-care (POC) settings has renewed interest. We prospectively screened at-risk individuals for SARS-CoV-2 spike and nucleocapsid protein antibodies in a POC setting to determine if it was a feasible method to identify antibody from prior infection. Three EUA-approved lateral flow antibody assays were performed on POC finger-stick blood and compared with serum and a CLIA nucleocapsid antibody immunoassay. Variables including antibody class, time since PCR, and the assay antigen used were evaluated. 512 subjects enrolled, of which 104 had a COVID-19 history and positive PCR. Only three PCR-positive subjects required hospitalization, with one requiring mechanical ventilation. The POC results correlated well with the immunoassay (93–97% sensitivity) and using serum did not improve the sensitivity or specificity. Finger-stick, POC COVID-19 antibody testing was highly effective in identifying antibody resulting from prior infections in mildly symptomatic subjects. Using high-complexity serum immunoassays did not improve the screening outcome. Almost all individuals with COVID-19 infection produced detectable antibodies to the virus. POC antibody testing is useful as a screen for prior COVID-19 infection, and should be useful in assessing vaccine response.
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DOI：
10.1007/s10096-020-04010-7
发表时间：
2020-08-17
影响因子：
4.5
作者：
Guedez-Lopez, Gladys Virginia;Alguacil-Guillen, Marina;Garcia-Rodriguez, Julio
通讯作者：
Garcia-Rodriguez, Julio
影响因子：
3.9
作者：
Pavlova, Ina P.;Nair, Sujit S.;Tewari, Ash K.
通讯作者：
Tewari, Ash K.
影响因子：
3.5
作者：
Manthei DM;Whalen JF;Schroeder LF;Sinay AM;Li SH;Valdez R;Giacherio DA;Gherasim C
通讯作者：
Gherasim C
影响因子：
12.4
作者：
Brigger D;Horn MP;Pennington LF;Powell AE;Siegrist D;Weber B;Engler O;Piezzi V;Damonti L;Iseli P;Hauser C;Froehlich TK;Villiger PM;Bachmann MF;Leib SL;Bittel P;Fiedler M;Largiadèr CR;Marschall J;Stalder H;Kim PS;Jardetzky TS;Eggel A;Nagler M
通讯作者：
Nagler M
DOI：
10.1056/nejmoa2034545
发表时间：
2021-02-11
期刊：
The New England journal of medicine
影响因子：
--
作者：
Lumley SF;O'Donnell D;Stoesser NE;Matthews PC;Howarth A;Hatch SB;Marsden BD;Cox S;James T;Warren F;Peck LJ;Ritter TG;de Toledo Z;Warren L;Axten D;Cornall RJ;Jones EY;Stuart DI;Screaton G;Ebner D;Hoosdally S;Chand M;Crook DW;O'Donnell AM;Conlon CP;Pouwels KB;Walker AS;Peto TEA;Hopkins S;Walker TM;Jeffery K;Eyre DW;Oxford University Hospitals Staff Testing Group
通讯作者：
Oxford University Hospitals Staff Testing Group