Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay
Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay
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DOI：
10.1128/aac.00550-19
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发表时间：
2019-07-01
影响因子：
4.9
通讯作者：
Spanua, Teresa
中科院分区：
文献类型：
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作者：
Giordano, Liliana;Fiori, Barbara;Spanua, Teresa
我们使用NG-Test Carba 5检测法直接检测了484种临床微生物（n = 310）和模拟（n = 174）阳性血液培养物，用于检测产碳青霉烯酶的肠杆菌。除4种KPC（170/171）、oxa -48样（22/22）、VIM（19/21）和NDM（14/15）产生者外，其余均无假阳性。在临床检测的肺炎克雷伯菌中，123株KPC菌中检出122株，1株oxa -48样菌中检出1株，2株VIM菌中检出1株。一些VIM和NDM生产商用该方法获得了少量但仍然可读的条带。没有生物产生该试验设计用于检测的IMPS。
We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.