OPTIMIZATION OF MICELLAR ELECTROKINETIC CHROMATOGRAPHY
OPTIMIZATION OF MICELLAR ELECTROKINETIC CHROMATOGRAPHY
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DOI：
10.1021/ac00212a019
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发表时间：
1990-07-01
影响因子：
7.4
通讯作者：
FOLEY, JP
中科院分区：
文献类型：
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作者：
FOLEY, JP
考察了胶束电动毛细管色谱(MEC)对中性溶质的分离能力。提出并评价了分辨率(fl，)和单位时间分辨率(ff？/Fr)的优化理论。导出了预测最佳保留因数(k‘)的方程，并导出了与分析时间有关的另一个方程：R“a(选择性)、ktjt^和H/Vk(板高度/电渗透速度)。最佳分辨率的容量因子由*‘=(fme/f0)1/2给出，其中f0和t^。分别是水相和胶束相的保留时间。单位时间最佳分辨率的容量因子为1.2~2。用单位时间最佳分辨率容量因子代替单位时间最佳分辨率容量因子所得到的分离，其分辨率可能稍差，但速度要快得多。在最佳分离条件下，MEC的分离质量与溶质疏水性TTy无关。讨论了表面活性剂梯度用于分离具有多种疏水分子的溶质的优点。并对MEC、胶束液相色谱和常规高效液相色谱进行了简要的比较。
The separating power of micellarelectrokinetic chromatography (MEC) for neutral solutes Is critically examined. A theory for the optimization of resolution (fl,) and resolution per unit time (ff «/fR) Is presented and evaluated. Equations are derived that predict the optimum retention factor (k') and the corresponding surfactant concentration to use Another equation Is derived that relates the analysis time to five pa-rameters: R „a (selectivity), ktjt^., and H/vK (plate helght/electroosmotlc velocity). The capacity factor for the best resolution Is given by*'=(fme/f0) 1/2, where f0 and t^. are the retention times of the aqueous and micellar phases, respectively. The capacity factor for the best resolution per unit time ranges from 1.2 to 2. The separations obtained by using the optimum capacity factor for resolution perunit time Instead of resolution may be slightly poorer In terms of reso-lution but are much faster. Provided the optimum conditions are employed, the quality of MEC separations Is Independent of the solute hydrophobic tty. The advantages of a surfactant gradient for the separation of solutes with a wide range of hydrophoblcttles are discussed. A brief comparison of MEC, micellar liquid chromatography, and conventional high-performance liquid chromatography Is also made.