3D imaging of undissected optically cleared Anopheles stephensi mosquitoes infected with Plasmodium parasites
3D imaging of undissected optically cleared Anopheles stephensi mosquitoes infected with Plasmodium parasites
复制标题
感染疟原虫寄生虫的未解剖光学透明史氏按蚊的 3D 成像
DOI：
10.1101/682054
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
F. Frischknecht
中科院分区：
文献类型：
--
作者：
Mariana De Niz;Jessica Kehrer;Nicolas M. B. Brancucci;F. Moalli;E. Reynaud;J. Stein;F. Frischknecht
疟疾是一种威胁生命的疾病，由疟原虫属的顶复门寄生虫引起。按蚊对于这些寄生虫的性复制及其传播给包括人类在内的脊椎动物宿主是必需的。已尝试使用多种显微镜方法对昆虫载体内的寄生虫进行成像，其中大多数方法受到蚊子的光散射不透明角质层的存在的阻碍。到目前为止，疟原虫阶段的成像取决于重要解剖部位的切片或手术解剖，如中肠和唾液腺。光学投影断层扫描（OPT）和光片荧光显微镜（LSFM）使成像领域的厘米级，同时提供微米分辨率。在本文中，我们提出了重建的整个身体的疟原虫感染，光学清除斯氏按蚊蚊子和他们的中肠。来自OPT成像的3D重建显示了蚊子解剖结构的详细特征，并使中肠寄生虫的整体定位成为可能。此外，蚊子中肠的LSFM成像显示了提取的中肠中卵囊的详细分布。
Malaria is a life-threatening disease, caused by Apicomplexan parasites of the Plasmodium genus. The Anopheles mosquito is necessary for the sexual replication of these parasites and for their transmission to vertebrate hosts, including humans. Imaging of the parasite within the insect vector has been attempted using multiple microscopy methods, most of which are hampered by the presence of the light scattering opaque cuticle of the mosquito. So far, imaging of the Plasmodium mosquito stages depended on either sectioning or surgical dissection of important anatomical sites, such as the midgut and the salivary glands. Optical projection tomography (OPT) and light sheet fluorescence microscopy (LSFM) enable imaging fields of view in the centimeter scale whilst providing micrometer resolution. In this paper, we present reconstructions of the whole body of Plasmodium-infected, optically cleared Anopheles stephensi mosquitoes and their midguts. The 3D-reconstructions from OPT imaging show detailed features of the mosquito anatomy and enable overall localization of parasites in midguts. Additionally, LSFM imaging of mosquito midguts shows detailed distribution of oocysts in extracted midguts.
影响因子：
48
作者：
Dodt, Hans-Ulrich;Leischner, Ulrich;Becker, Klaus
通讯作者：
Becker, Klaus
DOI：
--
发表时间：
1984
期刊：
The Journal of experimental biology
影响因子：
--
作者：
J. Ribeiro;P. Rossignol;A. Spielman
通讯作者：
J. Ribeiro;P. Rossignol;A. Spielman