Isolation and characterization of an autoinducer synthase from Acinetobacter baumannii
Isolation and characterization of an autoinducer synthase from Acinetobacter baumannii
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DOI：
10.1128/jb.01929-07
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发表时间：
2008-05-01
影响因子：
3.2
通讯作者：
Rather, Philip N.
中科院分区：
文献类型：
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作者：
Niu, Chen;Clemmer, Katy M.;Rather, Philip N.
利用根癌农杆菌（Agrobacterium tumefaciens）traG-lacZ生物传感器，研究了条件致病菌鲍曼不动杆菌（Acinetobacter baumannii）M2菌株产生的酰基高丝氨酸内酯（阿勒）信号。了全境克隆了鲍曼不动杆菌（baumannii）基因，命名为abaI，并在重组大肠杆菌中指导阿勒的生产。AbaI蛋白类似于自诱导物合酶的LuxI家族成员，并被预测为A.鲍曼不动杆菌。由AbaI引导的主要阿勒信号通过质谱鉴定为N-（3-羟基十二烷酰基）-L-HSL（3-羟基-C-12-HSL）。还发现了少量的至少另外5种AHLs。abaI在转录水平上的表达被培养上清液的乙酸乙酯提取物或合成的3-羟基-C-12-HSL激活。一个abaI：：Km突变体未能产生任何可检测的阿勒信号，并在生物膜发育受损。
The opportunistic human pathogen Acinetobacter baumannii strain M2 was found to produce distinct acyl-homoserine lactone (AHL) signals based on the use of an Agrobacterium tumefaciens traG-lacZ biosensor. An A. baumannii gene, designated abaI, was cloned and directed AHL production in recombinant Escherichia coli. The AbaI protein was similar to members of the LuxI family of autoinducer synthases and was predicted to be the only autoinducer synthase encoded by A. baumannii. The primary AHL signal directed by AbaI was identified by mass spectrometry as being N-(3-hydroxydodecanoyl)-L-HSL (3-hydroxy-C-12-HSL). Minor amounts of at least five additional AHLs were also identified. The expression of abaI at the transcriptional level was activated by ethyl acetate extracts of culture supernatants or by synthetic 3-hydroxy-C-12-HSL. An abaI::Km mutant failed to produce any detectable AHL signals and was impaired in biofilm development.