Altered Sensory Code Drives Juvenile-to-Adult Behavioral Maturation in Caenorhabditis elegans.
Altered Sensory Code Drives Juvenile-to-Adult Behavioral Maturation in Caenorhabditis elegans.
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DOI：
10.1523/eneuro.0175-16.2016
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发表时间：
2016-11
期刊：
影响因子：
3.4
通讯作者：
Chalasani SH
中科院分区：
文献类型：
--
作者：
Hale LA;Lee ES;Pantazis AK;Chronis N;Chalasani SH
在寻求食物的任务中，成人的表现要比少年表现更好。使用线虫秀丽隐杆线虫探测行为成熟的神经机制，我们发现成年人和少年需要不同的感觉神经元组合来产生特定年龄的寻求食物的行为。我们首先表明，成年人和少年对食物相关气味的反应和偏好有所不同，我们分析了遗传突变体以绘制这些行为反应所需的神经元回路。我们开发了一种新颖的装置来捕获少年并记录其神经元活性。活性测量表明，成人和少年AWA感觉神经元对添加二乙酰基刺激的响应，而AWB，询问和AWC感觉神经元专门在成人中编码其去除。此外，我们表明，从其他AWB中减少神经传递，询问，AWC感觉神经元将气味偏好从成年人转变为少年状态。我们还表明，AWB和问神经元仅在成年人中驱动行为变化，提供了更多证据表明特定年龄的电路推动了特定年龄的行为。总的来说，我们的结果表明，在动物发育中的少年到成人的过渡期间，对气味诱发的感觉代码进行了修改，以推动适合年龄的行为。我们建议这种改变的感官代码专门使成年人提取其他刺激特征并产生强大的行为。
Adults perform better than juveniles in food-seeking tasks. Using the nematode Caenorhabditis elegans to probe the neural mechanisms underlying behavioral maturation, we found that adults and juveniles require different combinations of sensory neurons to generate age-specific food-seeking behavior. We first show that adults and juveniles differ in their response to and preference for food-associated odors, and we analyze genetic mutants to map the neuronal circuits required for those behavioral responses. We developed a novel device to trap juveniles and record their neuronal activity. Activity measurements revealed that adult and juvenile AWA sensory neurons respond to the addition of diacetyl stimulus, whereas AWB, ASK, and AWC sensory neurons encode its removal specifically in adults. Further, we show that reducing neurotransmission from the additional AWB, ASK, and AWC sensory neurons transforms odor preferences from an adult to a juvenile-like state. We also show that AWB and ASK neurons drive behavioral changes exclusively in adults, providing more evidence that age-specific circuits drive age-specific behavior. Collectively, our results show that an odor-evoked sensory code is modified during the juvenile-to-adult transition in animal development to drive age-appropriate behavior. We suggest that this altered sensory code specifically enables adults to extract additional stimulus features and generate robust behavior.