Prion protein in sheep urine
Prion protein in sheep urine
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DOI：
10.1177/104063870802000201
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发表时间：
2008-03-01
影响因子：
1.5
通讯作者：
Nielsen, Klaus
中科院分区：
文献类型：
--
作者：
Andrievskaia, Olga;Algire, James;Nielsen, Klaus
细胞朊病毒蛋白 (PrP(C)) 的错误折叠形式是传染性海绵状脑病传染原的主要成分，也是这些疾病的经过验证的生物标志物。 PrP(C) 的表达在中枢神经系统中最高，并且已在外周组织中发现。脑脊液、尿液、血清、乳汁和精浆中均检测到可溶性 PrP(C)。在本研究中，尝试对来自正常和感染羊痒病的绵羊的朊病毒蛋白病尿液样本进行表征。收集来自感染瘙痒症的绵羊和年龄匹配的健康绵羊的尿液样本，并通过蛋白质印迹法进行浓度分析。使用抗 PrP 单克隆抗体对 PrP(C) 的 C 末端部分进行免疫印迹后，可以看到分子量约为 27-30 kDa 的蛋白酶 K 敏感蛋白条带。但仅用二抗对 PrP(C) 的所有 N 末端表位进行单克隆抗体免疫印迹后则不然。通过与免疫印迹中已知的绵羊重组 PrP 量进行比较，估计了 49 只动物（对照组：n = 16；自然瘙痒病感染组）：11 = 33）尿液中 PrP(C) 的量。尿液中 PrP(C) 的背景浓度为 0-0。 16 ng/ml（根据尿液样本的初始非浓缩体积进行调整）。 33 只自然感染痒病的动物中，有 7 只的尿液中 PrP(C) 水平均升高（0.3-4.7 ng/ml）。尿锌中 PrP(C) 的来源以及感染羊痒病的绵羊尿液中 PrP(C) 水平升高的原因尚待探索。
The misfolded form of cellular prion protein protein (PrP(C)) is the main component Of the infectious agent of transmissible spongiform encephalopathies and the validated biomarker for these diseases. The expression of PrP(C) is highest in the central nervous system and has been found in peripheral tissues. Soluble PrP(C) has been detected in cerebrospinal fluid, urine, serum, milk, and seminal plasma. In this study attempts were made to characterize prion protein ill urine S samples from normal and scrapie-infected sheep. Urine samples from scrapie-infected sheep and age-matched healthy sheep were collected and analyzed by Western blot following, concentration. A protease K-sensitive protein band with a molecular weight of approximately 27-30 kDa was visualized after immunoblotting with anti-PrP monoclonal antibodies to a C-terminal Part of PrP(C). but not after immunoblotting with monoclonal antibodies to ail N-terminal epitope of PrP(C) of with secondary antibodies only. The amount of PrP(C) in the urine of 49 animals (control group: n = 16; naturally scrapie-infected group): 11 = 33) was estimated by comparison with known amounts of ovine recombinant PrP in the immunoblot. Background concentration of PrP(C) in urine was found to be 0-0. 16 ng/ml (adjusted to the initial nonconcentrated volume of the urine samples). Seven out of 33 naturally scrapie-infected animals had ail elevated level (0.3-4.7 ng/ml) of PrP(C) in urine. The origin of PrP(C) in urine zinc the reason for the increased level of PrP(C) in scrapie-infected sheep urine has yet to be explored.