NONCHEMOTACTIC MUTANTS OF ESCHERICHIA COLI
NONCHEMOTACTIC MUTANTS OF ESCHERICHIA COLI
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DOI：
10.1128/jb.93.1.390-398.1967
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发表时间：
1967-01-01
影响因子：
3.2
通讯作者：
DAHL, MM
中科院分区：
文献类型：
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作者：
ARMSTRONG, JB;ADLER, J;DAHL, MM
我们已经分离出40株大肠杆菌的突变体，它们是非趋化性的，这是根据它们在半固体胰蛋白胨平板上不能群集或在含有胰蛋白胨肉汤的毛细管中不能形成条带来判断的。所有突变体的鞭毛形态及与抗鞭毛血清的反应均正常。在显微镜下它们都是完全能动的，并且对噬菌体chi都很敏感。不像它的父母，突变体之一，更详细的研究，未能显示趋化性对氧，葡萄糖，丝氨酸，苏氨酸，或天冬氨酸。未能表现出化学趋化因子并不是因为未能使用这些化学物质而造成的。该突变体的游动是随机的。在几种条件下生长速度正常，生长要求不变。
We have isolated 40 mutants of Esherichia coli which are nonchemotactic as judged by their failure to swarm on semisolid tryptone plates or to make bands in capillary tubes containing tryptone broth. All the mutants have normal flagella a fact shown by their shape and reaction with antiflagella serum. All are fully motile under the microscope and all are sensitive to the phage chi. Unlike its parent, one of the mutants, studied in greater detail, failed to show chemotaxis toward oxygen, glucose, serine, threonine, or aspartic acid. The failure to exhibit chemotaxts does not result from a failure to use the chemicals. The swimming of this mutant was shown to be random. The growth rate was normal under several conditions, and the growth requirements were unchanged.